TPDB

3D PDB MODEL

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TPDB ID TPDB72518

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 40-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

SDGRDAAANDKATDLIALTARRDPCCSNPVCTVHNPQICG

FASTA

>TPDB72518|Neurotoxin|length=40
SDGRDAAANDKATDLIALTARRDPCCSNPVCTVHNPQICG

Formula C168H278N56O60S4

Absent Amino Acids EMFWY

Common Amino Acids A

Mass 4170.63

PI 5.31

Basic Residues 5

Acidic Residues 5

Hydrophobic Residues 12

Net Charge -1

Boman Index 10.84

Hydrophobicity -0.390

Aliphatic Index 68.50

Half Life

Mammalian: 1.9 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 250

Absorbance 280nm 5.99

Polar Residues 13

number

6

A

3

R

3

N

5

D

4

C

0

E

1

Q

2

G

1

H

2

I

2

L

1

K

0

M

0

F

3

P

2

S

3

T

0

W

0

Y

2

V

Amino Acid Distribution

A: 6 R: 3 N: 3 D: 5 C: 4 E: 0 Q: 1 G: 2 H: 1 I: 2 L: 2 K: 1 M: 0 F: 0 P: 3 S: 2 T: 3 W: 0 Y: 0 V: 2

Length

40

Formula

C168H278N56O60S4

Mass

4170.63

PI

5.31

Net Charge

-1

Boman Index

10.84

Hydrophobicity

-0.390

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them (PubMed:13679412, PubMed:28917942). This toxin blocks mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4) when heterologously expressed in Xenopus oocytes (PubMed:13679412, PubMed:28917942). Does not block the muscle nor the major neuronal nAChR alpha-4/beta-2 (PubMed:13679412). Discriminates between nAChRs containing alpha-6 and alpha-3 subunits, being selective for alpha-6 (PubMed:13679412). Also reduces the frequency of responses from the giant fiber (GF)-dorsal longitudinal muscle pathway in the D.melanogaster GF circuit, but has no effect on the GF-tergo trochanteral muscle pathway (PubMed:28917942). {ECO:0000269|PubMed:13679412, ECO:0000269|PubMed:28917942}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus purpurascens (Purple cone)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Mechanism

Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them (PubMed:13679412, PubMed:28917942). This toxin blocks mammalian nAChRs (alpha-6 or -3/beta-2 or -3 > alpha-3/beta-2 > alpha-3/beta-4) when heterologously expressed in Xenopus oocytes (PubMed:13679412, PubMed:28917942).

Protein Name Alpha-conotoxin PIA

Venom Taxon Group cone snail

Protein Family Conotoxin A superfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Acetylcholine receptor inhibiting toxin Amidation Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:13679412, ECO:0000305|PubMed:28917942}.

Keywords

3D-structure Acetylcholine receptor inhibiting toxin Amidation Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin A superfamily

Protein_Name

Alpha-conotoxin PIA

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:13679412, ECO:0000305|PubMed:28917942}.

Venom_Taxon_Group

cone snail