TPDB

3D PDB MODEL

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TPDB ID TPDB72507

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 39-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

SDGRNVAAKAFHRIGRTIRDECCSNPACRVNNPHVCRRR

FASTA

>TPDB72507|Neurotoxin|length=39
SDGRNVAAKAFHRIGRTIRDECCSNPACRVNNPHVCRRR

Formula C179H300N72O53S4

Absent Amino Acids QLMWY

Common Amino Acids R

Mass 4437.04

PI 10.99

Basic Residues 11

Acidic Residues 3

Hydrophobic Residues 10

Net Charge +6

Boman Index 13.69

Hydrophobicity -0.910

Aliphatic Index 52.56

Half Life

Mammalian: 1.9 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 250

Absorbance 280nm 5.63

Polar Residues 11

number

4

A

8

R

4

N

2

D

4

C

1

E

0

Q

2

G

2

H

2

I

0

L

1

K

0

M

1

F

2

P

2

S

1

T

0

W

0

Y

3

V

Amino Acid Distribution

A: 4 R: 8 N: 4 D: 2 C: 4 E: 1 Q: 0 G: 2 H: 2 I: 2 L: 0 K: 1 M: 0 F: 1 P: 2 S: 2 T: 1 W: 0 Y: 0 V: 3

Length

39

Formula

C179H300N72O53S4

Mass

4437.04

PI

10.99

Net Charge

+6

Boman Index

13.69

Hydrophobicity

-0.910

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: [Alpha-conotoxin ArIA]: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin acts as a competitive inhibitor and is 3-fold more potent on alpha-7/CHRNA7 nAChRs (IC(50)=6 nM) than on alpha-3-beta-2/CHRNA3-CHRNB2 nAChR (IC(50)=18 nM). {ECO:0000269|PubMed:17497892}. FUNCTION: [Alpha-conotoxin ArIB]: Acts as a competitive inhibitor and is 33-fold more potent on alpha-7/CHRNA7 nAChRs (IC(50)=1.8 nM) than on alpha-3-beta-2/CHRNA3-CHRNB2 nAChR (IC(50)=60.1 nM). {ECO:0000269|PubMed:17497892}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus arenatus (Sand-dusted cone)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Mechanism

[Alpha-conotoxin ArIA]: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin acts as a competitive inhibitor and is 3-fold more potent on alpha-7/CHRNA7 nAChRs (IC(50)=6 nM) than on alpha-3-beta-2/CHRNA3-CHRNB2 nAChR (IC(50)=18 nM).. FUNCTION: [Alpha-conotoxin ArIB]: Acts as a competitive inhibitor and is 33-fold more potent on alpha-7/CHRNA7 nAChRs (IC(50)=1.8 nM) than on alpha-3-beta-2/CHRNA3-CHRNB2 nAChR (IC(50)=60.1 nM)..

Protein Name Alpha-conotoxin ArIA [Cleaved into: Alpha-conotoxin ArIB]

Venom Taxon Group cone snail

Protein Family Conotoxin A superfamily

Protein Existence Evidence at protein level

Keywords Acetylcholine receptor inhibiting toxin Disulfide bond Hydroxylation Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:17497892}.

Keywords

Acetylcholine receptor inhibiting toxin Disulfide bond Hydroxylation Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin A superfamily

Protein_Name

Alpha-conotoxin ArIA [Cleaved into: Alpha-conotoxin ArIB]

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:17497892}.

Venom_Taxon_Group

cone snail