TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB72502

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 50-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

AEDSRGTQLHRALRKATKLSESTRCKRKGSSCRRTSYDCCTGSCRNGKCG

FASTA

>TPDB72502|Neurotoxin|length=50
AEDSRGTQLHRALRKATKLSESTRCKRKGSSCRRTSYDCCTGSCRNGKCG

Formula C216H375N83O74S6

Absent Amino Acids IMFPWV

Common Amino Acids R

Mass 5511.23

PI 10.21

Basic Residues 14

Acidic Residues 4

Hydrophobic Residues 7

Net Charge +9

Boman Index 16.98

Hydrophobicity -1.206

Aliphatic Index 29.40

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1865

Absorbance 280nm 33.84

Polar Residues 21

number

3

A

8

R

1

N

2

D

6

C

2

E

1

Q

5

G

1

H

0

I

3

L

5

K

0

M

0

F

0

P

7

S

5

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 3 R: 8 N: 1 D: 2 C: 6 E: 2 Q: 1 G: 5 H: 1 I: 0 L: 3 K: 5 M: 0 F: 0 P: 0 S: 7 T: 5 W: 0 Y: 1 V: 0

Length

50

Formula

C216H375N83O74S6

Mass

5511.23

PI

10.21

Net Charge

+9

Boman Index

16.98

Hydrophobicity

-1.206

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav). This toxin selectively and potently inhibits depolarization-activated rat Cav2.2/CACNA1B currents (IC(50)=89 nM), when coexpressed with alpha-2/delta-1 (CACNA2D1) and beta-3 (CACNB3) subunits. In vivo, is lethal to fish and displays potent analgesic activity in mice pain models of hot plate and acetic acid writhing but has fewer side effects on mouse motor function and lower toxicity in goldfish. Shows higher or similar analgesic activity in the pain models mentioned above compared to MVIIA, and lower side effects. In addition, it blocks Cav2.2/CACNA1B more rapidly than MVIIA and also dissociates more rapidly. {ECO:0000269|PubMed:34589389}.

Target

Target or Channel voltage-gated calcium channel (Cav)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Conus bullatus (Bubble cone)

Neurotoxin Type Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Mechanism

Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav). This toxin selectively and potently inhibits depolarization-activated rat Cav2.2/CACNA1B currents (IC(50)=89 nM), when coexpressed with alpha-2/delta-1 (CACNA2D1) and beta-3 (CACNB3) subunits.

Protein Name Omega-conotoxin Bu8

Venom Taxon Group cone snail

Protein Family Conotoxin O1 superfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Amidation Calcium channel impairing toxin Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:21266071}.

Keywords

3D-structure Amidation Calcium channel impairing toxin Disulfide bond Ion channel impairing toxin Knottin Neurotoxin Secreted Signal Toxin Voltage-gated calcium channel impairing toxin

Neurotoxin_Type

Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Conotoxin O1 superfamily

Protein_Name

Omega-conotoxin Bu8

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:21266071}.

Venom_Taxon_Group

cone snail