TPDB

3D PDB MODEL

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TPDB ID TPDB72471

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 62-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MQVFYGLLLMFILCSTIHLSEQWCSTCLDLACGASRECYDPCFKAFGRAHGKCMNNKCRCYT

FASTA

>TPDB72471|Neurotoxin|length=62
MQVFYGLLLMFILCSTIHLSEQWCSTCLDLACGASRECYDPCFKAFGRAHGKCMNNKCRCYT

Formula C308H473N83O85S12

Absent Amino Acids

Common Amino Acids C

Mass 7083.37

PI 7.79

Basic Residues 8

Acidic Residues 4

Hydrophobic Residues 25

Net Charge +2

Boman Index 0.06

Hydrophobicity 0.218

Aliphatic Index 67.74

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 10470

Absorbance 280nm 147.81

Polar Residues 23

number

4

A

3

R

2

N

2

D

9

C

2

E

2

Q

4

G

2

H

2

I

7

L

3

K

3

M

4

F

1

P

4

S

3

T

1

W

3

Y

1

V

Amino Acid Distribution

A: 4 R: 3 N: 2 D: 2 C: 9 E: 2 Q: 2 G: 4 H: 2 I: 2 L: 7 K: 3 M: 3 F: 4 P: 1 S: 4 T: 3 W: 1 Y: 3 V: 1

Length

62

Formula

C308H473N83O85S12

Mass

7083.37

PI

7.79

Net Charge

+2

Boman Index

0.06

Hydrophobicity

0.218

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Potently blocks Kv1.3/KCNA3, Kv1.2/KCNA2, and Shaker potassium channels (PubMed:24590385) and inhibits high conductance calcium-activated potassium channels (PubMed:10082164). Is potent against Kv1.3/KCNN3 (IC(50)=0.55 nM) and Kv1.2/KCNN2 (IC(50)=6.19 nM) (PubMed:24590385). It also stimulates the release of NO, IL-6 and TNF in J774.1 cells and presents a pro-inflammatory activity in mice (PubMed:21549737, PubMed:23085190). {ECO:0000269|PubMed:10082164, ECO:0000269|PubMed:21549737, ECO:0000269|PubMed:23085190, ECO:0000269|PubMed:24590385}.

Target

Target or Channel voltage-gated potassium channel (Kv)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Tityus serrulatus (Brazilian yellow scorpion)

Neurotoxin Type Neurotoxin Calcium-activated potassium channel impairing toxin Ion channel impairing toxin Potassium channel impairing toxin

Mechanism

Potently blocks Kv1.3/KCNA3, Kv1.2/KCNA2, and Shaker potassium channels (PubMed:24590385) and inhibits high conductance calcium-activated potassium channels (PubMed:10082164). Is potent against Kv1.3/KCNN3 (IC(50)=0.55 nM) and Kv1.2/KCNN2 (IC(50)=6.19 nM) (PubMed:24590385).

Protein Name Potassium channel toxin alpha-KTx 12.1 (Butantoxin) (BuTX) (Tityustoxin-6) (Ts6) (TsTX-IV)

Venom Taxon Group scorpion

Protein Family Short scorpion toxin superfamily, Potassium channel inhibitor family, Alpha-KTx 12 subfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Calcium-activated potassium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Potassium channel impairing toxin Secreted Signal Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:25199494}.

Keywords

3D-structure Calcium-activated potassium channel impairing toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Potassium channel impairing toxin Secreted Signal Toxin

Neurotoxin_Type

Neurotoxin Calcium-activated potassium channel impairing toxin Ion channel impairing toxin Potassium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Short scorpion toxin superfamily, Potassium channel inhibitor family, Alpha-KTx 12 subfamily

Protein_Name

Potassium channel toxin alpha-KTx 12.1 (Butantoxin) (BuTX) (Tityustoxin-6) (Ts6) (TsTX-IV)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:25199494}.

Venom_Taxon_Group

scorpion