TPDB

3D PDB MODEL

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TPDB ID TPDB72398

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 67-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MASKIFFVLAVFLVMSAVLPESFAGCKKLNSYCTRQHRECCHGLVCRRPDYGIGRGILWKCTRARKG

FASTA

>TPDB72398|Neurotoxin|length=67
MASKIFFVLAVFLVMSAVLPESFAGCKKLNSYCTRQHRECCHGLVCRRPDYGIGRGILWKCTRARKG

Formula C336H540N100O84S8

Absent Amino Acids

Common Amino Acids R

Mass 7581.08

PI 10.07

Basic Residues 14

Acidic Residues 3

Hydrophobic Residues 28

Net Charge +9

Boman Index 5.63

Hydrophobicity 0.115

Aliphatic Index 81.49

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 8855

Absorbance 280nm 116.80

Polar Residues 16

number

5

A

7

R

1

N

1

D

6

C

2

E

1

Q

6

G

2

H

3

I

6

L

5

K

2

M

4

F

2

P

4

S

2

T

1

W

2

Y

5

V

Amino Acid Distribution

A: 5 R: 7 N: 1 D: 1 C: 6 E: 2 Q: 1 G: 6 H: 2 I: 3 L: 6 K: 5 M: 2 F: 4 P: 2 S: 4 T: 2 W: 1 Y: 2 V: 5

Length

67

Formula

C336H540N100O84S8

Mass

7581.08

PI

10.07

Net Charge

+9

Boman Index

5.63

Hydrophobicity

0.115

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Alpha-toxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin shows inhibition against mouse alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE) (IC(50)=1215 nM), rat alpha-3-beta-4/CHRNA3-CHRNB4 (IC(50)=5.173 uM), rat alpha-7/CHRNA7 (IC(50)=4.786 uM), human alpha-7/CHRNA7 (IC(50)=8.869 uM), and rat alpha-9-alpha-10/CHRNA9-CHRNA10 (IC(50)=202 nM). Also competes with alpha-bungarotoxin for binding to orthosteric sites on muscle-type T.carlifornicus (IC(50)=256 nM) and human alpha-7/CHRNA7 nAChRs (IC(50)=19.81 uM). {ECO:0000269|PubMed:36668848}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Metridium senile (Brown sea anemone) (Frilled sea anemone)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin

Mechanism

Alpha-toxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin shows inhibition against mouse alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE) (IC(50)=1215 nM), rat alpha-3-beta-4/CHRNA3-CHRNB4 (IC(50)=5.173 uM), rat alpha-7/CHRNA7 (IC(50)=4.786 uM), human alpha-7/CHRNA7 (IC(50)=8.869 uM), and rat alpha-9-alpha-10/CHRNA9-CHRNA10 (IC(50)=202 nM).

Protein Name Alpha-actitoxin-Ms11a-3 (Alpha-AITX-Ms11a-3) (Alpha-anmTX-Ms11a-3)

Venom Taxon Group sea anemone

Protein Existence Evidence at protein level

Keywords 3D-structure Acetylcholine receptor inhibiting toxin Amidation Direct protein sequencing Disulfide bond Knottin Nematocyst Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin

Keywords

3D-structure Acetylcholine receptor inhibiting toxin Amidation Direct protein sequencing Disulfide bond Knottin Nematocyst Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin

Protein_Existence

Evidence at protein level

Protein_Name

Alpha-actitoxin-Ms11a-3 (Alpha-AITX-Ms11a-3) (Alpha-anmTX-Ms11a-3)

Venom_Taxon_Group

sea anemone