TPDB

3D PDB MODEL

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TPDB ID TPDB72275

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 334-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MMNLKYLLFFCLVQALHYCYAYGDPSLSNELDRFNPCPYSDDTVKMIILTRENKKHDFYTLNTIKNHNEFKKSTIKHQVVFITHGFTSTATAENFLAMAEALLDKGNYLVILIDWRVAACTNEMAGVKLAYYSYAASNTRLVGNYIATVTKMLVQQYNVPMANIRLIGHSLGAHTSGFAGKKVQELRLGKYSEIIGLDPAGPSFKSQECSQRICETDANYVQIIHTSNHLGTLVTLGTVDFYMNNGYNQPGCGLPIIGETCSHTRAVKYFTECIRHECCLIGVPQSKNPQPVSKCTRNECVCVGLNAKTYPKTGSFYVPVESKAPYCNNKGKII

FASTA

>TPDB72275|Neurotoxin|length=334
MMNLKYLLFFCLVQALHYCYAYGDPSLSNELDRFNPCPYSDDTVKMIILTRENKKHDFYTLNTIKNHNEFKKSTIKHQVVFITHGFTSTATAENFLAMAEALLDKGNYLVILIDWRVAACTNEMAGVKLAYYSYAASNTRLVGNYIATVTKMLVQQYNVPMANIRLIGHSLGAHTSGFAGKKVQELRLGKYSEIIGLDPAGPSFKSQECSQRICETDANYVQIIHTSNHLGTLVTLGTVDFYMNNGYNQPGCGLPIIGETCSHTRAVKYFTECIRHECCLIGVPQSKNPQPVSKCTRNECVCVGLNAKTYPKTGSFYVPVESKAPYCNNKGKII

Formula C1664H2597N445O484S23

Absent Amino Acids

Common Amino Acids L

Mass 37318.00

PI 8.33

Basic Residues 44

Acidic Residues 25

Hydrophobic Residues 135

Net Charge +8

Boman Index 47.81

Hydrophobicity -0.146

Aliphatic Index 82.90

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 36175

Absorbance 280nm 96.94

Polar Residues 114

number

22

A

10

R

24

N

10

D

15

C

15

E

11

Q

22

G

11

H

21

I

28

L

23

K

8

M

13

F

14

P

19

S

25

T

1

W

20

Y

22

V

Amino Acid Distribution

A: 22 R: 10 N: 24 D: 10 C: 15 E: 15 Q: 11 G: 22 H: 11 I: 21 L: 28 K: 23 M: 8 F: 13 P: 14 S: 19 T: 25 W: 1 Y: 20 V: 22

Length

334

Formula

C1664H2597N445O484S23

Mass

37318.00

PI

8.33

Net Charge

+8

Boman Index

47.81

Hydrophobicity

-0.146

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Catalyzes the hydrolysis of phosphatidylcholine with phospholipase A1 activity (3.6 U/ml) (PubMed:23159790). May act as an allergen and induce hemolytic activity (By similarity). In vivo, a mixture of this protein and Ves a 1.02 is able to paralyze crickets (PubMed:23159790). {ECO:0000250|UniProtKB:P0DMB7, ECO:0000269|PubMed:23159790}.

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Vespa affinis (Lesser banded hornet)

Neurotoxin Type Neurotoxin

Mechanism

Catalyzes the hydrolysis of phosphatidylcholine with phospholipase A1 activity (3.6 U/ml) (PubMed:23159790). May act as an allergen and induce hemolytic activity (By similarity).

Protein Name Phospholipase A1 1 (PLA1) (EC 3.1.1.32) (Allergen Ves a 1.01) (Vespapase) (allergen Vesp a 1)

Venom Taxon Group insect

Protein Family AB hydrolase superfamily, Lipase family

Protein Existence Evidence at protein level

Keywords Allergen Cytolysis Disulfide bond Hemolysis Hydrolase Lipid degradation Lipid metabolism Neurotoxin Secreted Signal Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:23159790}.

Keywords

Allergen Cytolysis Disulfide bond Hemolysis Hydrolase Lipid degradation Lipid metabolism Neurotoxin Secreted Signal Toxin

Neurotoxin_Type

Neurotoxin

Protein_Existence

Evidence at protein level

Protein_Family

AB hydrolase superfamily, Lipase family

Protein_Name

Phospholipase A1 1 (PLA1) (EC 3.1.1.32) (Allergen Ves a 1.01) (Vespapase) (allergen Vesp a 1)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:23159790}.

Venom_Taxon_Group

insect