TPDB

3D PDB MODEL

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TPDB ID TPDB72241

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 111-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MKTLLLAVAVVAFVCLGSADQLGLGRQQIDWGKGQAKGPPYTLCFECNRETCSNCFKDNRCPPYHRTCYTLYRPDGNGEMKWAVKGCAKTCPTAQPGESVQCCNTPKCNDY

FASTA

>TPDB72241|Neurotoxin|length=111
MKTLLLAVAVVAFVCLGSADQLGLGRQQIDWGKGQAKGPPYTLCFECNRETCSNCFKDNRCPPYHRTCYTLYRPDGNGEMKWAVKGCAKTCPTAQPGESVQCCNTPKCNDY

Formula C527H822N150O158S14

Absent Amino Acids

Common Amino Acids C

Mass 12236.07

PI 8.02

Basic Residues 14

Acidic Residues 9

Hydrophobic Residues 35

Net Charge +4

Boman Index 17.51

Hydrophobicity -0.422

Aliphatic Index 54.50

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 19200

Absorbance 280nm 156.91

Polar Residues 40

number

8

A

5

R

6

N

5

D

12

C

4

E

6

Q

10

G

1

H

1

I

8

L

8

K

2

M

3

F

8

P

3

S

8

T

2

W

5

Y

6

V

Amino Acid Distribution

A: 8 R: 5 N: 6 D: 5 C: 12 E: 4 Q: 6 G: 10 H: 1 I: 1 L: 8 K: 8 M: 2 F: 3 P: 8 S: 3 T: 8 W: 2 Y: 5 V: 6

Length

111

Formula

C527H822N150O158S14

Mass

12236.07

PI

8.02

Net Charge

+4

Boman Index

17.51

Hydrophobicity

-0.422

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: This bird and reptile-specific postsynaptic neurotoxin inhibits the chick muscle alpha-1-beta-1-gamma-delta (CHRNA1-CHRNB1-CHRNG-CHRND) nicotinic acetylcholine receptor (nAChR) 100-fold more compared with the mouse receptor. In vivo, produces rapid flaccid paralysis, dyspnea and increased respiratory rate in geckos. At sublethal doses geckos were immobilized for up to three days and then recovered. Chicks injected with lethal doses showed rapid onset of inactivity, dyspnea and neck droop, and no extended paralysis with survival was seen. {ECO:0000269|PubMed:18952712}.

Target

Target or Channel nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Boiga irregularis (Brown tree snake) (Coluber irregularis)

Neurotoxin Type Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Mechanism

This bird and reptile-specific postsynaptic neurotoxin inhibits the chick muscle alpha-1-beta-1-gamma-delta (CHRNA1-CHRNB1-CHRNG-CHRND) nicotinic acetylcholine receptor (nAChR) 100-fold more compared with the mouse receptor. In vivo, produces rapid flaccid paralysis, dyspnea and increased respiratory rate in geckos.

Protein Name Irditoxin subunit B (IrTxB)

Venom Taxon Group snake

Protein Family Three-finger toxin family, Ancestral subfamily, Boigatoxin sub-subfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Pyrrolidone carboxylic acid Secreted Signal Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.

Keywords

3D-structure Acetylcholine receptor inhibiting toxin Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Pyrrolidone carboxylic acid Secreted Signal Toxin

Neurotoxin_Type

Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Three-finger toxin family, Ancestral subfamily, Boigatoxin sub-subfamily

Protein_Name

Irditoxin subunit B (IrTxB)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.

Venom_Taxon_Group

snake