TPDB

3D PDB MODEL

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TPDB ID TPDB72187

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 183-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MKFSLFFSVFFLAVLHACLSESEIDLEDEEHFMSSDSFLSEIQDESRGKTCIERNKECTNDRHGCCRGKIFKDKCTCVKNGKTEKCVCTQKKWAKIIESYIGDIPALPKPVDDKCVPKHADCSKRKDDCCKGGIFKYQCKCYDMYDDDGEKTDLCGCVSPVEHQAIEGALRIAKKLIGDRWGR

FASTA

>TPDB72187|Neurotoxin|length=183
MKFSLFFSVFFLAVLHACLSESEIDLEDEEHFMSSDSFLSEIQDESRGKTCIERNKECTNDRHGCCRGKIFKDKCTCVKNGKTEKCVCTQKKWAKIIESYIGDIPALPKPVDDKCVPKHADCSKRKDDCCKGGIFKYQCKCYDMYDDDGEKTDLCGCVSPVEHQAIEGALRIAKKLIGDRWGR

Formula C900H1423N249O277S20

Absent Amino Acids

Common Amino Acids K

Mass 20804.90

PI 6.52

Basic Residues 36

Acidic Residues 32

Hydrophobic Residues 56

Net Charge -1

Boman Index 60.30

Hydrophobicity -0.538

Aliphatic Index 63.93

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 17960

Absorbance 280nm 86.33

Polar Residues 46

number

8

A

8

R

3

N

18

D

17

C

14

E

4

Q

12

G

5

H

12

I

10

L

23

K

3

M

9

F

5

P

12

S

6

T

2

W

4

Y

8

V

Amino Acid Distribution

A: 8 R: 8 N: 3 D: 18 C: 17 E: 14 Q: 4 G: 12 H: 5 I: 12 L: 10 K: 23 M: 3 F: 9 P: 5 S: 12 T: 6 W: 2 Y: 4 V: 8

Length

183

Formula

C900H1423N249O277S20

Mass

20804.90

PI

6.52

Net Charge

-1

Boman Index

60.30

Hydrophobicity

-0.538

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Spider venom toxin that exhibits cytolytic activity by forming an alpha-helix across the membrane (PubMed:20657014). Lethal to insect larvae (PubMed:20657014, PubMed:24717175). Causes instant paralysis and death in the larvae of the flesh fly (S.carnaria) at doses of 20 ug/g, at doses of less than 10 ug/g causes reversible paralysis (PubMed:20657014). Has cytolytic activity against insect Sf9 cells (PubMed:20657014). Causes stable and irreversible depolarization of fly muscle fibers, leading to contracture at higher toxin concentrations (PubMed:20657014). Destabilizes membranes (PubMed:20657014). {ECO:0000269|PubMed:20657014, ECO:0000269|PubMed:24717175}.

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Cheiracanthium punctorium (Yellow sac spider) (Aranea punctoria)

Neurotoxin Type Neurotoxin

Mechanism

Spider venom toxin that exhibits cytolytic activity by forming an alpha-helix across the membrane (PubMed:20657014). Lethal to insect larvae (PubMed:20657014, PubMed:24717175).

Protein Name DELTA-miturgitoxin-Cp1a (DELTA-MGTX-Cp1a) (Double-knot toxin) (DkTx) (Toxin CpTx1) (Toxin CpTx1a)

Venom Taxon Group spider

Protein Family Neurotoxin 19 (CSTX) family, Double-CSTX subfamily

Protein Existence Evidence at protein level

Keywords Amidation Cytolysis Direct protein sequencing Disulfide bond Knottin Membrane Neurotoxin Repeat Secreted Signal Target cell membrane Target membrane Toxin Transmembrane

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:20657014}.

Keywords

Amidation Cytolysis Direct protein sequencing Disulfide bond Knottin Membrane Neurotoxin Repeat Secreted Signal Target cell membrane Target membrane Toxin Transmembrane

Neurotoxin_Type

Neurotoxin

Protein_Existence

Evidence at protein level

Protein_Family

Neurotoxin 19 (CSTX) family, Double-CSTX subfamily

Protein_Name

DELTA-miturgitoxin-Cp1a (DELTA-MGTX-Cp1a) (Double-knot toxin) (DkTx) (Toxin CpTx1) (Toxin CpTx1a)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:20657014}.

Venom_Taxon_Group

spider