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Peptide record

TPDB72122

Neurotoxin standard
86 amino acids
Basic Information
3D PDB MODEL
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TPDB72122
Neurotoxin
Toxicity and safety peptides
NTxPred2
standard
No
A 86-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.
Sequence
MTRFVLFISCFFLIDMIVECKKEGYLVGNDGCKYGCITRPHQYCVHECELKKGTDGYCAYWLACYCYNMPDWVKTWSSATNKCKGK
Physicochemical Analysis
C449H672N112O122S13
C
10007.73
7.95
13
8
34
+3
5.41
-0.127
61.16
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
27555
275.34
29
Residue Composition
number
3
A
2
R
3
N
4
D
10
C
4
E
1
Q
7
G
2
H
4
I
5
L
9
K
3
M
4
F
2
P
3
S
5
T
3
W
7
Y
5
V
Amino Acid Distribution
A: 3 R: 2 N: 3 D: 4 C: 10 E: 4 Q: 1 G: 7 H: 2 I: 4 L: 5 K: 9 M: 3 F: 4 P: 2 S: 3 T: 5 W: 3 Y: 7 V: 5
Chemical Descriptors
86
C449H672N112O122S13
10007.73
7.95
+3
5.41
-0.127
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: Beta toxin that show multiple effects. It enhances the open probability at more negative potentials of human Nav1.3/SCN3A and Nav1.6/SCN8A, of the insect channel BgNaV1 and of arachnid VdNaV1 channel (PubMed:30463697). It promotes an important shift in slow inactivation processes as a function of the prepulse voltage in human Nav1.3/SCN3A and Nav1.6/SCN8A and a small shift in Nav1.1/SCN1A, Nav1.2/SCN2A and Nav1.4/SCN4A (PubMed:30463697). Finally, it reduces the peak of sodium currents in Nav1.3/SCN3A (80% inhibition at 70 nM of toxin), Nav1.6/SCN8A (55.3%), Nav1.1/SCN1A (53.3%), Nav1.5/SCN5A (46.7%), Nav1.2/SCN2A (42.7%) and Nav1.4/SCN4A (20%) voltage-gated sodium channels (PubMed:30463697). It has also been shown to affect the sodium current permeability of rat cerebellum granular cells in a partially reversible manner (PubMed:11821128). In vivo, an intraperitoneal injection (20 ug) into mice produces excitability, respiratory problems, convulsions and death, within the first 30 minutes after injection (PubMed:11821128). {ECO:0000269|PubMed:11821128, ECO:0000269|PubMed:30463697}.

Target

voltage-gated sodium channel (Nav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Tityus obscurus (Amazonian scorpion) (Tityus cambridgei)
Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin
Beta toxin that show multiple effects. It enhances the open probability at more negative potentials of human Nav1.3/SCN3A and Nav1.6/SCN8A, of the insect channel BgNaV1 and of arachnid VdNaV1 channel (PubMed:30463697).
Beta-mammal/insect toxin To1 (NaTx12.1) (Toxin Tc49b)
scorpion
Long (4 C-C) scorpion toxin superfamily, Sodium channel inhibitor family, Beta subfamily
Evidence at protein level
Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:11821128}.
Additional Detail Fields 7 fields
Amidation Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Secreted Signal Toxin Voltage-gated sodium channel impairing toxin
Neurotoxin Ion channel impairing toxin Voltage-gated sodium channel impairing toxin
Evidence at protein level
Long (4 C-C) scorpion toxin superfamily, Sodium channel inhibitor family, Beta subfamily
Beta-mammal/insect toxin To1 (NaTx12.1) (Toxin Tc49b)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:11821128}.
scorpion