TPDB

3D PDB MODEL

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TPDB ID TPDB72111

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 61-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MKAFYGMLVIFILCSTCYISVDSQIDTNVKCSGSSKCVKICIDRYNTRGAKCINGRCTCYP

FASTA

>TPDB72111|Neurotoxin|length=61
MKAFYGMLVIFILCSTCYISVDSQIDTNVKCSGSSKCVKICIDRYNTRGAKCINGRCTCYP

Formula C292H473N79O86S10

Absent Amino Acids EHW

Common Amino Acids C

Mass 6787.05

PI 8.50

Basic Residues 8

Acidic Residues 3

Hydrophobic Residues 23

Net Charge +5

Boman Index 2.99

Hydrophobicity 0.252

Aliphatic Index 79.84

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 6460

Absorbance 280nm 95.18

Polar Residues 26

number

2

A

3

R

3

N

3

D

8

C

0

E

1

Q

4

G

0

H

7

I

2

L

5

K

2

M

2

F

1

P

6

S

4

T

0

W

4

Y

4

V

Amino Acid Distribution

A: 2 R: 3 N: 3 D: 3 C: 8 E: 0 Q: 1 G: 4 H: 0 I: 7 L: 2 K: 5 M: 2 F: 2 P: 1 S: 6 T: 4 W: 0 Y: 4 V: 4

Length

61

Formula

C292H473N79O86S10

Mass

6787.05

PI

8.50

Net Charge

+5

Boman Index

2.99

Hydrophobicity

0.252

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: Irreversibly blocks the A-type voltage-gated potassium channels in rat cerebellum granular cells (190 nM induce 50% inhibitory effect) (IC(50)=190 nM) (PubMed:15369825, PubMed:18280256). Also weakly inhibits Kv1.2/KCNA2 and Kv1.3/KCNA3 (PubMed:29483648). {ECO:0000269|PubMed:15369825, ECO:0000269|PubMed:18280256, ECO:0000269|PubMed:29483648}.

Target

Target or Channel voltage-gated potassium channel (Kv)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Tityus discrepans (Venezuelan scorpion)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Mechanism

Irreversibly blocks the A-type voltage-gated potassium channels in rat cerebellum granular cells (190 nM induce 50% inhibitory effect) (IC(50)=190 nM) (PubMed:15369825, PubMed:18280256). Also weakly inhibits Kv1.2/KCNA2 and Kv1.3/KCNA3 (PubMed:29483648)..

Protein Name Potassium channel toxin alpha-KTx 15.6 (Discrepin)

Venom Taxon Group scorpion

Protein Family Short scorpion toxin superfamily, Potassium channel inhibitor family, Alpha-KTx 15 subfamily

Protein Existence Evidence at protein level

Keywords 3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Potassium channel impairing toxin Pyrrolidone carboxylic acid Secreted Signal Toxin Voltage-gated potassium channel impairing toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:15369825}.

Keywords

3D-structure Direct protein sequencing Disulfide bond Ion channel impairing toxin Neurotoxin Potassium channel impairing toxin Pyrrolidone carboxylic acid Secreted Signal Toxin Voltage-gated potassium channel impairing toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin Voltage-gated potassium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Short scorpion toxin superfamily, Potassium channel inhibitor family, Alpha-KTx 15 subfamily

Protein_Name

Potassium channel toxin alpha-KTx 15.6 (Discrepin)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:15369825}.

Venom_Taxon_Group

scorpion