TPDB

3D PDB MODEL

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TPDB ID TPDB72062

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 703-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MSSDLVMPALGRPFTLGMLYDARREKLIPGFSLFGDETLQKYQSSNAQRSSEFKIVASDSTESKSSAMDIEASLGVSFLGGLVEVGGSAKYLNNTKKYQNQSRVTLKYKATTVYKQFTAPPGTVTVQETAITEKGLATHVVTSILYGANAFFVSDSDKVEDTNLQDIQGKMEAAIKKIPTISIEGSASVQLTDEEKSLASNLSCKFHGDFLLESLPTTFEDAVKTYQTLPTLIGEDGANSVPMKVWLAPLKSYNSKAQQLIQEINVSKVRRIHTTLEELHKLKRRANEAMDVKLVQRIPLIHDKISNFQQIFQDYMLTVQKKIAEKLPLVRAGTESEQSLQKIIDDRAQSPFSNEKVSKWLDAVEREIAVLKSCAGMVEGTQAKFVSNQTELDREVLVGKVKHAVCFIFTSVERNDPYLKVLSDYWESPPSNNAKDVAPSTEDKWCFSTEVVLKMQQRAQTFCDHVNDFEKSRNVGFFITALENGKFQGASIYYYKEGSLATQDFTFPRMPFVQGYKKRSDLLWYACDLTFDRNTINNWISLSDNDTFAASEHGKRQNYPKHPERFVSFNQVLCNEGLMGKHYWEVEWNGYIDVGIAYISIPRKEIDFASAFGYNTYSWVLSYNPKIGYIERHKKREYNVRAPNPGFKRLGLFLDWRYGSISFYAVSSDEVHHLHTFKTKFTEPVYPAFSIGPAGNHGTLRLL

FASTA

>TPDB72062|Neurotoxin|length=703
MSSDLVMPALGRPFTLGMLYDARREKLIPGFSLFGDETLQKYQSSNAQRSSEFKIVASDSTESKSSAMDIEASLGVSFLGGLVEVGGSAKYLNNTKKYQNQSRVTLKYKATTVYKQFTAPPGTVTVQETAITEKGLATHVVTSILYGANAFFVSDSDKVEDTNLQDIQGKMEAAIKKIPTISIEGSASVQLTDEEKSLASNLSCKFHGDFLLESLPTTFEDAVKTYQTLPTLIGEDGANSVPMKVWLAPLKSYNSKAQQLIQEINVSKVRRIHTTLEELHKLKRRANEAMDVKLVQRIPLIHDKISNFQQIFQDYMLTVQKKIAEKLPLVRAGTESEQSLQKIIDDRAQSPFSNEKVSKWLDAVEREIAVLKSCAGMVEGTQAKFVSNQTELDREVLVGKVKHAVCFIFTSVERNDPYLKVLSDYWESPPSNNAKDVAPSTEDKWCFSTEVVLKMQQRAQTFCDHVNDFEKSRNVGFFITALENGKFQGASIYYYKEGSLATQDFTFPRMPFVQGYKKRSDLLWYACDLTFDRNTINNWISLSDNDTFAASEHGKRQNYPKHPERFVSFNQVLCNEGLMGKHYWEVEWNGYIDVGIAYISIPRKEIDFASAFGYNTYSWVLSYNPKIGYIERHKKREYNVRAPNPGFKRLGLFLDWRYGSISFYAVSSDEVHHLHTFKTKFTEPVYPAFSIGPAGNHGTLRLL

Formula C3577H5546N950O1066S19

Absent Amino Acids

Common Amino Acids S

Mass 79524.25

PI 7.89

Basic Residues 99

Acidic Residues 82

Hydrophobic Residues 279

Net Charge +3

Boman Index 147.56

Hydrophobicity -0.390

Aliphatic Index 79.05

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 98585

Absorbance 280nm 123.97

Polar Residues 205

number

47

A

29

R

33

N

36

D

7

C

46

E

32

Q

39

G

15

H

36

I

58

L

55

K

12

M

38

F

28

P

61

S

43

T

10

W

29

Y

49

V

Amino Acid Distribution

A: 47 R: 29 N: 33 D: 36 C: 7 E: 46 Q: 32 G: 39 H: 15 I: 36 L: 58 K: 55 M: 12 F: 38 P: 28 S: 61 T: 43 W: 10 Y: 29 V: 49

Length

703

Formula

C3577H5546N950O1066S19

Mass

79524.25

PI

7.89

Net Charge

+3

Boman Index

147.56

Hydrophobicity

-0.390

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: This lethal (towards mammals) heterodimer induces hemolytic activities due to its ability to form pores in the cell membrane (PubMed:12177053, PubMed:1790672, PubMed:26627714, PubMed:9271089). The pore may be composed of 10 SNTX-alpha/beta heterodimers (PubMed:26627714). The toxin elicits potent hypotension which is endothelium-dependent and appears to be mediated by the nitric oxide pathway and activation of potassium channels (PubMed:11931843, PubMed:8310447). In addition, it displays edema-inducing activities, increases vascular permeability. It also shows myotoxic activities and interferes irreversibly with neuromuscular function (PubMed:8079369). It also induces irreversible platelet aggregation in rabbit or rat (but not in human or mouse) whole blood (PubMed:8533137). In addition, it has been observed to increase spontaneous quantal acetylcholine release from isolated frog cutaneous pectoris motor endings (PubMed:8921306). {ECO:0000269|PubMed:11931843, ECO:0000269|PubMed:1790672, ECO:0000269|PubMed:26627714, ECO:0000269|PubMed:8079369, ECO:0000269|PubMed:8921306, ECO:0000269|PubMed:9271089}.

Target

Target or Channel voltage-gated potassium channel (Kv)

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Synanceia horrida (Estuarine stonefish) (Scorpaena horrida)

Neurotoxin Type Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin

Mechanism

This lethal (towards mammals) heterodimer induces hemolytic activities due to its ability to form pores in the cell membrane (PubMed:12177053, PubMed:1790672, PubMed:26627714, PubMed:9271089). The pore may be composed of 10 SNTX-alpha/beta heterodimers (PubMed:26627714).

Protein Name

Stonustoxin subunit alpha (SNTX subunit alpha) (DELTA-synanceitoxin-Sh1a) (DELTA-SYTX-Sh1a) (Trachynilysin subunit alpha) (TLY subunit alpha)

Venom Taxon Group other

Protein Family SNTX/VTX toxin family

Protein Existence Evidence at protein level

Keywords 3D-structure Cytolysis Direct protein sequencing Disulfide bond Hemolysis Hemostasis impairing toxin Ion channel impairing toxin Myotoxin Neurotoxin Platelet aggregation activating toxin Potassium channel impairing toxin Secreted Toxin

Tissue Specificity TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:8810331}.

Keywords

3D-structure Cytolysis Direct protein sequencing Disulfide bond Hemolysis Hemostasis impairing toxin Ion channel impairing toxin Myotoxin Neurotoxin Platelet aggregation activating toxin Potassium channel impairing toxin Secreted Toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin Potassium channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

SNTX/VTX toxin family

Protein_Name

Stonustoxin subunit alpha (SNTX subunit alpha) (DELTA-synanceitoxin-Sh1a) (DELTA-SYTX-Sh1a) (Trachynilysin subunit alpha) (TLY subunit alpha)

Tissue_Specificity

TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:8810331}.

Venom_Taxon_Group

other