TPDB

3D PDB MODEL

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TPDB ID TPDB71974

Activity

Neurotoxin

Activity_category_statistics

Toxicity and safety peptides

Database_Source

NTxPred2

Sequence_Type

standard

Has_Modification No

Description A 78-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.

Sequence

MKGTFLICLILIAGFSFKSTQAGSICLEPKVVGPCTAYFRRFYFDSETGKCTVFIYGGCEGNGNNFETLRACRAICRA

FASTA

>TPDB71974|Neurotoxin|length=78
MKGTFLICLILIAGFSFKSTQAGSICLEPKVVGPCTAYFRRFYFDSETGKCTVFIYGGCEGNGNNFETLRACRAICRA

Formula C383H593N101O106S8

Absent Amino Acids HW

Common Amino Acids G

Mass 8565.01

PI 8.42

Basic Residues 9

Acidic Residues 5

Hydrophobic Residues 32

Net Charge +4

Boman Index 2.27

Hydrophobicity 0.301

Aliphatic Index 73.85

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 4845

Absorbance 280nm 56.57

Polar Residues 24

number

6

A

5

R

3

N

1

D

7

C

4

E

1

Q

9

G

0

H

6

I

5

L

4

K

1

M

8

F

2

P

4

S

6

T

0

W

3

Y

3

V

Amino Acid Distribution

A: 6 R: 5 N: 3 D: 1 C: 7 E: 4 Q: 1 G: 9 H: 0 I: 6 L: 5 K: 4 M: 1 F: 8 P: 2 S: 4 T: 6 W: 0 Y: 3 V: 3

Length

78

Formula

C383H593N101O106S8

Mass

8565.01

PI

8.42

Net Charge

+4

Boman Index

2.27

Hydrophobicity

0.301

Evidence 1 Activity

Activity

Detail Source Activity Neurotoxin

Function Text

FUNCTION: This protease inhibitor shows two different activities, it inhibits both the capsaicin receptor TRPV1 and serine proteases. It partially (max 50%) and reversibly inhibits capsaicin-induced response of TRPV1 (IC(50)=54 nM), a receptor of the pain pathway (PubMed:18579526, PubMed:24351908). The second activity is a weak inhibition of trypsin and chymotrypsin activity (Ki=1 uM and Ki=5 uM, respectively) (PubMed:18579526). In vivo, it shows antinociceptive and analgesic activities (PubMed:24351908). It significantly prolongs tail-flick latency and reduces capsaicin-induced acute pain (PubMed:18579526, PubMed:24351908). In vivo, unlike other TRPV1 antagonists whose activity is associated with hyperthermia, this protein has the remarkable feature of dropping core body temperature (PubMed:24351908). {ECO:0000269|PubMed:18579526, ECO:0000269|PubMed:22982418, ECO:0000269|PubMed:24351908}.

Target

Target or Channel TRP channel

Source & Reference

Database Source NA

Source UniProtKB/Swiss-Prot Tox-Prot

Other

Organism Source Radianthus crispa (Leathery sea anemone) (Heteractis crispa)

Neurotoxin Type Neurotoxin Ion channel impairing toxin

Mechanism

This protease inhibitor shows two different activities, it inhibits both the capsaicin receptor TRPV1 and serine proteases. It partially (max 50%) and reversibly inhibits capsaicin-induced response of TRPV1 (IC(50)=54 nM), a receptor of the pain pathway (PubMed:18579526, PubMed:24351908).

Protein Name TauPI-stichotoxin-Hcr2b (TauPI-SHTX-Hcr2b) (Analgesic polypeptide HC1) (APHC1)

Venom Taxon Group sea anemone

Protein Family Venom Kunitz-type family, Sea anemone type 2 potassium channel toxin subfamily

Protein Existence Evidence at protein level

Keywords Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Protease inhibitor Secreted Serine protease inhibitor Signal Toxin

Keywords

Direct protein sequencing Disulfide bond Ion channel impairing toxin Nematocyst Neurotoxin Protease inhibitor Secreted Serine protease inhibitor Signal Toxin

Neurotoxin_Type

Neurotoxin Ion channel impairing toxin

Protein_Existence

Evidence at protein level

Protein_Family

Venom Kunitz-type family, Sea anemone type 2 potassium channel toxin subfamily

Protein_Name

TauPI-stichotoxin-Hcr2b (TauPI-SHTX-Hcr2b) (Analgesic polypeptide HC1) (APHC1)

Venom_Taxon_Group

sea anemone