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Peptide record

TPDB71963

Neurotoxin standard
95 amino acids
Basic Information
3D PDB MODEL
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TPDB71963
Neurotoxin
Toxicity and safety peptides
NTxPred2
standard
No
A 95-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.
Sequence
MPKLAVVLLVLLILPLSYFDAAGGQAVQGDWRGNRLARDLQRGGRDDESECIINTRDSPWGRCCRTRMCGSMCCPRNGCTCVYHWRRGHGCSCPG
Physicochemical Analysis
C444H711N145O128S13
RG
10545.20
8.32
15
8
31
+5
12.37
-0.277
69.79
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
20105
190.66
26
Residue Composition
number
5
A
12
R
3
N
6
D
10
C
2
E
3
Q
12
G
2
H
3
I
9
L
1
K
3
M
1
F
5
P
5
S
3
T
3
W
2
Y
5
V
Amino Acid Distribution
A: 5 R: 12 N: 3 D: 6 C: 10 E: 2 Q: 3 G: 12 H: 2 I: 3 L: 9 K: 1 M: 3 F: 1 P: 5 S: 5 T: 3 W: 3 Y: 2 V: 5
Chemical Descriptors
95
C444H711N145O128S13
10545.20
8.32
+5
12.37
-0.277
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: [Alpha-conotoxin VxXXB]: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Through its two C-terminal domains, this homodimeric protein would bind to two nAChR allosteric sites, located outside the nAChR C-loop of the principal binding face and at the adjacent binding interface in a clockwise direction (PubMed:37124228). It specifically blocks mammalian neuronal nAChR of the alpha-7/CHRNA7 (IC(50)=0.4 nM), alpha-3-beta-2/CHRNA3-CHRNB2 (IC(50)=8.4 nM) and alpha-4-beta-2/CHRNA4-CHRNB2 (IC(50)=228 nM) subtypes (PubMed:16790424). It inhibits alpha-7/CHRNA7, alpha-3-beta-2/CHRNA3-CHRNB2 and alpha-4-beta-2/CHRNA4-CHRNB2 nAChR subtypes more efficiently than VxXXA and VxXXC (PubMed:16790424). {ECO:0000269|PubMed:16790424, ECO:0000269|PubMed:37124228}.

Target

nicotinic acetylcholine receptor (nAChR) acetylcholine receptor

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Conus vexillum (Flag cone)
Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin
[Alpha-conotoxin VxXXB]: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Through its two C-terminal domains, this homodimeric protein would bind to two nAChR allosteric sites, located outside the nAChR C-loop of the principal binding face and at the adjacent binding interface in a clockwise direction (PubMed:37124228).
Alpha-conotoxin VxXXB (Alpha-D-conotoxin VxXXB) (Vx20.2) (VxXIIB) [Cleaved into: Alpha-conotoxin [des-Gly95]VxXXB]
cone snail
Conotoxin D superfamily
Evidence at protein level
3D-structure Acetylcholine receptor inhibiting toxin Amidation Direct protein sequencing Disulfide bond Gamma-carboxyglutamic acid Hydroxylation Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin
TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:16790424}.
Additional Detail Fields 7 fields
3D-structure Acetylcholine receptor inhibiting toxin Amidation Direct protein sequencing Disulfide bond Gamma-carboxyglutamic acid Hydroxylation Ion channel impairing toxin Neurotoxin Postsynaptic neurotoxin Secreted Signal Toxin
Neurotoxin Postsynaptic neurotoxin Acetylcholine receptor inhibiting toxin Ion channel impairing toxin
Evidence at protein level
Conotoxin D superfamily
Alpha-conotoxin VxXXB (Alpha-D-conotoxin VxXXB) (Vx20.2) (VxXIIB) [Cleaved into: Alpha-conotoxin [des-Gly95]VxXXB]
TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305|PubMed:16790424}.
cone snail