Back to search

Peptide record

TPDB71922

Neurotoxin standard
122 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB71922
Neurotoxin
Toxicity and safety peptides
NTxPred2
standard
No
A 122-aa standard natural neurotoxin peptide sequence curated from NTxPred2, with an available 3D structural model.
Sequence
MKVLIISAVLFITIFSNISAEIEDDFLEDESFEAEDIIPFFENEQARSCIPKHEECTNDKHNCCRKGLFKLKCQCSTFDDESGQPTERCACGRPMGHQAIETGLNIFRGLFKGKKKNKKTKG
Physicochemical Analysis
C604H956N166O186S10
WY
EK
13839.84
5.90
21
20
38
-2
41.25
-0.493
67.21
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
500
3.61
31
Residue Composition
number
6
A
5
R
6
N
7
D
8
C
13
E
4
Q
8
G
3
H
11
I
7
L
13
K
2
M
10
F
4
P
7
S
6
T
0
W
0
Y
2
V
Amino Acid Distribution
A: 6 R: 5 N: 6 D: 7 C: 8 E: 13 Q: 4 G: 8 H: 3 I: 11 L: 7 K: 13 M: 2 F: 10 P: 4 S: 7 T: 6 W: 0 Y: 0 V: 2
Chemical Descriptors
122
C604H956N166O186S10
13839.84
5.90
-2
41.25
-0.493
Evidence Records 1 records
Evidence 1 Activity

Activity

Neurotoxin
FUNCTION: [Toxin CSTX-1]: Spider venom toxin that shows calcium channel blocking activity and exhibits cytolytic activity by affecting the outer leaflet curvature and/or pore formation across the membrane (PubMed:22613721, PubMed:32290562). It blocks L-type calcium channels (Cav1/CACNA1) in mammalian neurons at nanomolar concentrations. Furthermore, it produces a slow voltage-independent block of mid/low and high voltage-activated calcium channels in cockroach neurons (PubMed:17517422). Potassium ions, histamine, M-ctenitoxin-Cs1a (AC P83619), CSTX-9 (AC P58604), and CSTX-13 (AC P83919) synergistically increase the insecticidal activity of this toxin (PubMed:15914655, PubMed:32290562). In vivo, it causes paralysis in blow flies and provokes death in drosophila (PubMed:32290562). {ECO:0000269|PubMed:17517422, ECO:0000269|PubMed:22613721, ECO:0000269|PubMed:32290562}. FUNCTION: [Toxin CSTX-2a]: Blocks voltage-activated calcium channels (Cav) (By similarity). Does not induce cell membrane permeability increase when tested on Xenopus oocytes. No alpha-helical structures are detectable. Is 7-fold less neurotoxic than omega-ctenitoxin-Cs1a on drosophila flies. {ECO:0000250}. FUNCTION: [Toxin CSTX-2b]: Blocks voltage-activated calcium channels (Cav) (By similarity). Is 190-fold less neurotoxic than omega-ctenitoxin-Cs1a on drosophila flies. {ECO:0000250}.

Target

voltage-gated calcium channel (Cav)

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Cupiennius salei (American wandering spider)
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin
[Toxin CSTX-1]: Spider venom toxin that shows calcium channel blocking activity and exhibits cytolytic activity by affecting the outer leaflet curvature and/or pore formation across the membrane (PubMed:22613721, PubMed:32290562). It blocks L-type calcium channels (Cav1/CACNA1) in mammalian neurons at nanomolar concentrations.
Toxin CSTX-1 (Omega-ctenitoxin-Cs1a) (Omega-CNTX-Cs1a) [Cleaved into: Toxin CSTX-2a (ctenitoxin-Cs2a) Toxin CSTX-2b (Ctenitoxin-Cs2b)]
spider
Neurotoxin 19 (CSTX) family, 04 (U1-Lctx) subfamily
Evidence at protein level
Amidation Calcium channel impairing toxin Cytolysis Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Membrane Neurotoxin Secreted Signal Target cell membrane Target membrane Toxin Voltage-gated calcium channel impairing toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:10772256}.
Additional Detail Fields 7 fields
Amidation Calcium channel impairing toxin Cytolysis Direct protein sequencing Disulfide bond Ion channel impairing toxin Knottin Membrane Neurotoxin Secreted Signal Target cell membrane Target membrane Toxin Voltage-gated calcium channel impairing toxin
Neurotoxin Calcium channel impairing toxin Ion channel impairing toxin Voltage-gated calcium channel impairing toxin
Evidence at protein level
Neurotoxin 19 (CSTX) family, 04 (U1-Lctx) subfamily
Toxin CSTX-1 (Omega-ctenitoxin-Cs1a) (Omega-CNTX-Cs1a) [Cleaved into: Toxin CSTX-2a (ctenitoxin-Cs2a) Toxin CSTX-2b (Ctenitoxin-Cs2b)]
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|PubMed:10772256}.
spider