TPDB

3D PDB MODEL

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TPDB ID TPDB64952

Peptide_Name

ASA-ApoB

Activity

Blood-Brain Barrier

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

BBPpredict

Sequence_Type

standard

Has_Modification No

Description A 38-aa standard natural blood-brain barrier peptide sequence curated from BBPpredict, with an available 3D structural model.

Modification

N-terminal: Conjugated with ASA drug

Sequence

SVIDALQYKLEGTTRLTRKRGLKLATALSLSNKFVEGS

FASTA

>TPDB64952|Blood-Brain Barrier|length=38
SVIDALQYKLEGTTRLTRKRGLKLATALSLSNKFVEGS

Formula C184H315N53O56

Absent Amino Acids CHMPW

Common Amino Acids L

Mass 4165.85

PI 10.62

Basic Residues 7

Acidic Residues 3

Hydrophobic Residues 15

Net Charge +4

Boman Index 7.10

Hydrophobicity -0.195

Aliphatic Index 105.26

Half Life

Mammalian: 1.9 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 35.77

Polar Residues 11

number

3

A

3

R

1

N

1

D

0

C

2

E

1

Q

3

G

0

H

1

I

7

L

4

K

0

M

1

F

0

P

4

S

4

T

0

W

1

Y

2

V

Amino Acid Distribution

A: 3 R: 3 N: 1 D: 1 C: 0 E: 2 Q: 1 G: 3 H: 0 I: 1 L: 7 K: 4 M: 0 F: 1 P: 0 S: 4 T: 4 W: 0 Y: 1 V: 2

Length

38

Formula

C184H315N53O56

Mass

4165.85

PI

10.62

Net Charge

+4

Boman Index

7.10

Hydrophobicity

-0.195

Conformation

Linear

N_terminal_Modification

Conjugated with ASA drug

Peptide_Length

Similarity

Peptide_Sequence_3Letter

SerValIleAspAlaLeuGlnTyrLysLeuGluGlyThrThrArgLeuThrArgLysArgGlyLeuLysLeuAlaThrAlaLeuSerLeuSerAsnLysPheValGluGlySer

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line CHO cell line

Assay & Model

In Vitro Concentration 2.5 microgram/ ml

In Vitro Method cellular uptake assay

In Vitro Result 41 % cellular uptake

Animal Model Origin/Source

Physical Condition Lysosomal disorder

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transendocytosis

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line bEND cell line

Assay & Model

In Vitro Concentration 2.5 microgram/ ml

In Vitro Method cellular uptake assay

In Vitro Result 15.5 % cellular uptake

Animal Model Origin/Source

Physical Condition Lysosomal disorder

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transendocytosis

Evidence 3 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line PBCECs cell line

Assay & Model

In Vitro Concentration 0.02% cells were added

In Vitro Method Basolateral screening assay

In Vitro Result 200% transfer

Animal Model Origin/Source

Physical Condition Lysosomal disorder

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Basolateral