TPDB

3D PDB MODEL

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TPDB ID TPDB64856

Peptide_Name

gH625

Activity

Blood-Brain Barrier

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

B3Pred BBPpredict Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 23-aa standard natural blood-brain barrier peptide sequence curated from B3Pred, BBPpredict, and Peptipedia, with an available 3D structural model.

Modification

Chemical: Chemically synthesized

Sequence

HGLASTLTRWAHYNALIRAFGGG

FASTA

>TPDB64856|Blood-Brain Barrier|length=23
HGLASTLTRWAHYNALIRAFGGG

Formula C112H169N35O29

Absent Amino Acids DCEQKMPV

Common Amino Acids AG

Mass 2469.79

PI 11.16

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +2

Boman Index -2.18

Hydrophobicity 0.043

Aliphatic Index 85.22

Half Life

Mammalian: 3.5 hour Yeast: 10 min E.coli: >10 hour

Extinction Coefficient Cystines 6990

Absorbance 280nm 283.02

Polar Residues 5

number

4

A

2

R

1

N

0

D

0

C

0

E

0

Q

4

G

2

H

1

I

3

L

0

K

0

M

1

F

0

P

1

S

2

T

1

W

1

Y

0

V

Amino Acid Distribution

A: 4 R: 2 N: 1 D: 0 C: 0 E: 0 Q: 0 G: 4 H: 2 I: 1 L: 3 K: 0 M: 0 F: 1 P: 0 S: 1 T: 2 W: 1 Y: 1 V: 0

Length

23

Formula

C112H169N35O29

Mass

2469.79

PI

11.16

Net Charge

+2

Boman Index

-2.18

Hydrophobicity

0.043

Chemical_Modification

Chemically synthesized

Conformation

Linear

Peptide_Length

Similarity

Peptide_Sequence_3Letter

HisGlyLeuAlaSerThrLeuThrArgTrpAlaHisTyrAsnAlaLeuIleArgAlaPheGlyGlyGly

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Human neuroblastoma cell line SH-SY5Y

Assay & Model

In Vitro Concentration 5*10^4 cells/well

In Vitro Method Spectorcluorometric analysis

In Vitro Result 20% fluoroscence measured at 1 micromolar peptide concentration

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transcytosis

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Human glioblastoma cell line U-87

Assay & Model

In Vitro Concentration 5*10^4 cells/well

In Vitro Method Spectorcluorometric analysis

In Vitro Result 35% fluoroscence measured at 1 micromolar peptide concentration

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transcytosis

Evidence 3 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Human neuroblastoma cell line SH-SY5Y

Assay & Model

In Vitro Concentration 5*10^4 cells/well

In Vitro Method Spectorcluorometric analysis

In Vitro Result 80% fluoroscence measured at 1 micromolar peptide concentration

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transcytosis

Evidence 4 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Human glioblastoma cell line U-87

Assay & Model

In Vitro Concentration 5*10^4 cells/well

In Vitro Method Spectorcluorometric analysis

In Vitro Result 90% fluoroscence measured at 1 micromolar peptide concentration

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type Transcytosis