TPDB

3D PDB MODEL

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TPDB ID TPDB64792

Peptide_Name

Retro-enantiomeric p

Activity

Blood-Brain Barrier

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

B3Pred BBPpredict Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 12-aa standard natural blood-brain barrier peptide sequence curated from B3Pred, BBPpredict, and Peptipedia, with an available 3D structural model.

Modification

C-terminal: Amidated

Sequence

PWVPSWMPPRHT

FASTA

>TPDB64792|Blood-Brain Barrier|length=12
PWVPSWMPPRHT

Formula C71H99N19O15S1

Absent Amino Acids ANDCEQGILKFY

Common Amino Acids P

Mass 1490.75

PI 11.05

Basic Residues 2

Acidic Residues 0

Hydrophobic Residues 4

Net Charge +1

Boman Index -0.02

Hydrophobicity -0.942

Aliphatic Index 24.17

Half Life

Mammalian: >20 hour Yeast: >20 hour E.coli: ?

Extinction Coefficient Cystines 11000

Absorbance 280nm 737.89

Polar Residues 2

number

0

A

1

R

0

N

0

D

0

C

0

E

0

Q

0

G

1

H

0

I

0

L

0

K

1

M

0

F

4

P

1

S

1

T

2

W

0

Y

1

V

Amino Acid Distribution

A: 0 R: 1 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 1 I: 0 L: 0 K: 0 M: 1 F: 0 P: 4 S: 1 T: 1 W: 2 Y: 0 V: 1

Length

12

Formula

C71H99N19O15S1

Mass

1490.75

PI

11.05

Net Charge

+1

Boman Index

-0.02

Hydrophobicity

-0.942

C_terminal_Modification

Amidated

Conformation

Linear

Peptide_Length

Similarity

Peptide_Sequence_3Letter

ProTrpValProSerTrpMetProProArgHisThr

Evidence 1 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Rat astrocyte

Assay & Model

In Vitro Concentration 50 micromolar

In Vitro Method HPLC, MALDI-TOF to analyze the peptide transport and apparent permeability

In Vitro Result 21.5+/- 2.8 apparent permeability

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type can transport cargo

Evidence 2 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Rat astrocyte

Assay & Model

In Vitro Concentration 50 micromolar

In Vitro Method HPLC, MALDI-TOF to analyze the peptide transport and apparent permeability

In Vitro Result 25.1+/- 3.0 apparent permeability

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type can transport cargo

Evidence 3 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Rat astrocyte

Assay & Model

In Vitro Concentration 50 micromolar

In Vitro Method PAMPA method for cargo delivery and transportation efficiency

In Vitro Result 0.27+/- 0.03 apparent permeability

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type can transport cargo

Combination Quantum dots

Evidence 4 Activity

Activity

Detail Source Activity Blood-Brain Barrier

Target

Cell Line Rat astrocyte

Assay & Model

In Vitro Concentration 50 micromolar

In Vitro Method PAMPA method for cargo delivery and transportation efficiency

In Vitro Result 0.031+/- 0.02 transport efficiency

Animal Model Origin/Source

Source & Reference

Database Source NA

Source B3Pdb

Other

Transport Type can transport cargo

Combination Quantum dots