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Peptide record

TPDB64561

TatNepNoCPP Cell-penetrating Peptides standard
23 amino acids
Basic Information
3D PDB MODEL
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TPDB64561
TatNepNoCPP
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 23-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
Chemical: neprilysin recognition sequence (Nep) and non-CPP peptide (DDDDEE) N-terminal: Conjugated with TAMRA
Sequence
YGRKKRRQRRRFFGFLADDDDEE
Physicochemical Analysis
C128H198N44O38
NCHIMPSTWV
R
2961.25
10.18
8
6
6
+2
11.34
-2.061
21.30
Mammalian: 2.8 hour Yeast: 10 min E.coli: 2 min
1490
50.32
2
Residue Composition
number
1
A
6
R
0
N
4
D
0
C
2
E
1
Q
2
G
0
H
0
I
1
L
2
K
0
M
3
F
0
P
0
S
0
T
0
W
1
Y
0
V
Amino Acid Distribution
A: 1 R: 6 N: 0 D: 4 C: 0 E: 2 Q: 1 G: 2 H: 0 I: 0 L: 1 K: 2 M: 0 F: 3 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0
Chemical Descriptors
23
C128H198N44O38
2961.25
10.18
+2
11.34
-2.061
Free
neprilysin recognition sequence (Nep) and non-CPP peptide (DDDDEE)
L
Linear
Conjugated with TAMRA
N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)O
Evidence Records 1 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

S. Cerevisiae (Mata And Matα ), Hek293T , Hek293T-Nep And HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Chimeric
TAMRA dye
TatNepNoCPP was 3 times higher than TatNep and 8.8 times higher than D-TatNepNoCPP. Internalization preference was HeLa > HEK293T > HEK293T-NEP
In vitro
CTTTTTTGGGTCCC
Additional Detail Fields 1 fields
CTTTTTTGGGTCCC