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Peptide record

TPDB64376

RALA Cell-penetrating Peptides standard
30 amino acids
Basic Information
3D PDB MODEL
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TPDB64376
RALA
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 MLCPP 2.0 Peptipedia PerseuCPP
standard
No
A 30-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, MLCPP 2.0, Peptipedia, and other sources, with an available 3D structural model.
Sequence
WEARLARALARALARHLARALARALRACEA
Physicochemical Analysis
C144H248N54O35S1
NDQGIKMFPSTYV
A
3327.95
12.28
8
2
19
+5
3.18
0.143
118.00
Mammalian: 2.8 hour Yeast: 3 min E.coli: 2 min
5500
165.27
1
Residue Composition
number
12
A
7
R
0
N
0
D
1
C
2
E
0
Q
0
G
1
H
0
I
6
L
0
K
0
M
0
F
0
P
0
S
0
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 12 R: 7 N: 0 D: 0 C: 1 E: 2 Q: 0 G: 0 H: 1 I: 0 L: 6 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0
Chemical Descriptors
30
C144H248N54O35S1
3327.95
12.28
+5
3.18
0.143
Free
L
Cationic and amphipathic Cationic Amphipathic
Linear
Free
N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)O N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CS)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 6 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

ZR-75-1, PC-3 and NCTC-929

Assay & Model

Female C57BL/6 mice
Female C57BL/6 mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic and amphipathic
Synthetic
Nanoparticle (Cy3-labeled nanoparticles complexed with DNA)
Nucleus
RALA/pDNA nanoparticles in vitro is comparable with commercial transfecting agents while exhibiting significantly lower cytotoxicity
Endocytic pathway
In vitro and in vivo
CCSTTHHHHHHHHHCCHHHHHHTSSCSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, A549, And HEK-293T Cell Line

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
5'Cy3-modified non-target siRNA and pCMV-Enhanced Green Fluorescent Protein (EGFP) plasmid DNA
pDNA transfection of RALA group was above 60% in HeLa cells.
More than one endocytosis pathways
In vitro
CCSTTHHHHHHHHHCCHHHHHHTSSCSCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
FITC-labelled p53-encoding plasmid DNA (pDNA)
Nucleus
RALA/pDNA carrier N/P ratios of 5 and 10 are internalized at a higher extent than N/P ratio of 2
In vitro
CCSTTHHHHHHHHHCCHHHHHHTSSCSCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

Hek293T And Ct26.Cl25 Cell Lines

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphipathic
5-methoxyuridine-modified mCherry mRNA, Enhanced green fluorescence protein (EGFP) mRNA and Ovalbumin (OVA) mRNA
Cytoplasm
4.9, 56.3 and 28.9% of mCherry-positive cells and 14.6, 86.5 and 29.5% of EGFP-positive cells were observed in the STR-Arg8, p5RHH and RALA complexes treatment groups, respectively in CT26.CL25 cancer cells.
In vitro
CCGGGCHHHHHHHHHSSCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

Hek-293 And Dc 2.4 Cells

Assay & Model

Male C57 BL/6 mice (6-8 weeks old)
Male C57 BL/6 mice (6-8 weeks old)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphipathic
Fusogenic
GFP-tagged pPSCA plasmid
Complex pPSCA to RALA resulted in significantly higher transfection efficacies at N:P ratios 4 and 10, in HEK-293 cells and DC 2.4 cells respectively, compared to using naked pPSCA (N:P 0) alone.
In vitro and in vivo
CCSTTHHHHHHHHHCCHHHHHHTSSCSCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

Nctc-929 Fibroblast Cells

Assay & Model

Tumour-bearing C57BL/6 mice
Tumour-bearing C57BL/6 mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
E6/E7 DNA and Nanoparticles (NPs)
RALA-E6/E7 NPs successfully transfected NCTC-929 cells as demonstrated by over expression of both HPV-16 E6 and HPV-16 E7 antigens compared to untreated cells
In vitro and in vivo
CCSSSCCTTCCCCCSCC
Additional Detail Fields 1 fields
CCSTTHHHHHHHHHCCHHHHHHTSSCSCC CCGGGCHHHHHHHHHSSCC CCSSSCCTTCCCCCSCC