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Peptide record

TPDB64335

PepH1 Blood-Brain Barrier Cell-penetrating Peptides standard
10 amino acids
Basic Information
3D PDB MODEL
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TPDB64335
PepH1
Blood-Brain Barrier Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
B3Pred BBPpredict Peptipedia CPPsite3.0
standard
No
A 10-aa standard natural multi-activity (Blood-Brain Barrier and Cell-penetrating Peptides) peptide sequence curated from B3Pred, BBPpredict, Peptipedia, and other sources, with an available 3D structural model.
Sequence
VQQLTKRFSL
Physicochemical Analysis
C55H94N16O15
ANDCEGHIMPWY
QL
1219.45
11.49
2
0
4
+2
1.05
-0.230
107.00
Mammalian: 100 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
4
Residue Composition
number
0
A
1
R
0
N
0
D
0
C
0
E
2
Q
0
G
0
H
0
I
2
L
1
K
0
M
1
F
0
P
1
S
1
T
0
W
0
Y
1
V
Amino Acid Distribution
A: 0 R: 1 N: 0 D: 0 C: 0 E: 0 Q: 2 G: 0 H: 0 I: 0 L: 2 K: 1 M: 0 F: 1 P: 0 S: 1 T: 1 W: 0 Y: 0 V: 1
Chemical Descriptors
10
C55H94N16O15
1219.45
11.49
+2
1.05
-0.230
Free
L
Linear
Hydrophobic
Linear
Free
Similarity
Cationic
ValGlnGlnLeuThrLysArgPheSerLeu
N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)O N[C@@H](Cc1ccccc1)C(=O)/N=C/C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O
Evidence Records 5 records
Evidence 1 Activity

Activity

Blood-Brain Barrier

Target

bEND cell line

Assay & Model

5 microCi/ml
Gamma counter radioactivity measure for TcPz
73.83+/- 9.40
Origin/Source

Source & Reference

NA
B3Pdb

Other

Derived from dengue virus capsid
Can cross blood brain barrier as well as shows membrane adsorption
Transmigration
Can be migrated to liver, kidney etc.
Evidence 2 Activity

Activity

Blood-Brain Barrier

Target

bEND cell line

Assay & Model

5 microCi/ml
Gamma counter radioactivity measure for gaNODA
71.47+/- 3.70
Origin/Source

Source & Reference

NA
B3Pdb

Other

Derived from dengue virus capsid
Can cross blood brain barrier as well as shows membrane adsorption
Transmigration
Can be migrated to liver, kidney etc.
Evidence 3 Activity

Activity

Blood-Brain Barrier
73.83%

Assay & Model

Initial concentration in donor chamber-Culture
Origin/Source
Translocated

Source & Reference

NA
B3Pdb

Other

Chemically synthesized
Permeability
99mTc(CO)3+
Evidence 4 Activity

Activity

Blood-Brain Barrier
0.02 %/g

Assay & Model

Origin/Source
% injected dose

Source & Reference

NA
B3Pdb

Other

Chemically synthesized
Intravenous
Permeability
99mTc(CO)3+
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

bEnd.3 Cell

Assay & Model

CD1 mice
CD1 mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Hydrophobic
Protein derived
Radiolabelled using fac-[99mTc(CO)3(H2O)3]+ and 67-Ga-labeled
PepH1 had minimal internalization and membrane retention (0.12±0.00 and 0.38±0.10 %) was able to transmigrate the BEB, with up to 73.8±9.4 %.
In vitro and in vivo
CCTTSSCCCCC
Additional Detail Fields 2 fields
CCTTSSCCCCC
Intravenous