TPDB

3D PDB MODEL

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TPDB ID TPDB64333

Peptide_Name

AcPHF6R9 T-peptide

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 15-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

C-terminal: Amidation N-terminal: Acetylation

Sequence

Ac-VQIVYKRRRRRRRRR-NH2

FASTA

>TPDB64333|Cell-penetrating Peptides|length=15
Ac-VQIVYKRRRRRRRRR-NH2

Formula C90H168N44O18

Absent Amino Acids ANDCEGHLMFPSTW

Common Amino Acids R

Mass 2154.61

PI 12.95

Basic Residues 10

Acidic Residues 0

Hydrophobic Residues 4

Net Charge +10

Boman Index 7.75

Hydrophobicity -2.420

Aliphatic Index 64.67

Half Life

Mammalian: 100 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 69.15

Polar Residues 2

number

0

A

9

R

0

N

0

D

0

C

0

E

1

Q

0

G

0

H

1

I

0

L

1

K

0

M

0

F

0

P

0

S

0

T

0

W

1

Y

2

V

Amino Acid Distribution

A: 0 R: 9 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 0 H: 0 I: 1 L: 0 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 2

Length

15

Formula

C90H168N44O18

Mass

2154.61

PI

12.95

Net Charge

+10

Boman Index

7.75

Hydrophobicity

-2.420

C_terminal_Modification

Amidation

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Acetylation

SMILES

N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Ht-22 Or HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Non-natural residues Linear L Cationic

Source of Peptide Fusogenic

Cargo Type Rhodamine labeled

Subcellular Localization Cytoplasm, Nucleus and Lysosomes

Uptake Efficiency Approx. 3 times more Rh-T-peptide entered cells in the first 3 h compared to Rh-PHF6.

In Vitro or In Vivo In vitro

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Ht-22 , HeLa And Hek293 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Rhodamine-labelled at N terminus

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency

Punctate staining in and around the nucleus and diffuse staining of the cytosol was observed in all cell types treated with RhPHF6R9 and 3 times more in the first 3 h relative to RhPHF6.

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCHHHHTCCC

DSSP_Secondary_Structure

CCCCHHHHTCCC