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Peptide record

TPDB64032

[(RW)5K](RW)5 Cell-penetrating Peptides modified_plain modified
21 amino acids
Basic Information
3D PDB MODEL
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Sequence-matched coordinates; source-recorded chemical modifications are not verified in this model.

TPDB64032
[(RW)5K](RW)5
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
modified_plain
Yes
A 21-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
N-terminal: Conjugated with FITC
Sequence
RWRWRWRWRWKRWRWRWRWRW
Physicochemical Analysis

Sequence-only estimates are not provided for modified or non-standard source notation. Reported molecular properties, when available, are retained below with their source records.

Chemical Descriptors
21 main sequence record
Free Activity
L Activity
Hybrid (Cyclic and Linear) Activity
Conjugated with FITC Activity
N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)O Activity
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

CCRF-CEM, Sk-Ov-3, Hek-293 And MDA-MB-231 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Hybrid (Cyclic and Linear) L
Synthetic
Carboxyfluorescein-labeled phosphopeptide (F′-GpYEEI)
Cytosol
[(RW)5K](RW)5 (10 μM) enhanced the cellular uptake of F′-GpYEEI (2 μM) by 18-fold in CCRF-CEM cells.
Direct penetration and clathrin-mediated endocytosis
In vitro
CCCCSSCCCSSCSSCCSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

CCRF-CEM, Sk-Ov-3, Hek-293 And MDA-MB-231 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Hybrid (Cyclic and Linear) L
Synthetic
Carboxyfluorescein-labeled anti-HIV drugs (lamivudine (F′-3TC), emtricitabine (F′-FTC), Stavudine (F′-d4T))
Cytosol
[(RW)5K](RW)5 (10 μM) significantly increased the cellular uptake of F′-3TC, F′-FTC, F′-d4T (2μM) in CCRF-CEM by 4.3-, 7-, and 7.6-fold, respectively,
Direct penetration and clathrin-mediated endocytosis
In vitro
CCCCSSCCCSSCSSCCSCC
Additional Detail Fields 1 fields
CCCCSSCCCSSCSSCCSCC