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Peptide record

TPDB64032

[(RW)5K](RW)5 Cell-penetrating Peptides standard
21 amino acids
Basic Information
3D PDB MODEL
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TPDB64032
[(RW)5K](RW)5
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 21-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
N-terminal: Conjugated with FITC
Sequence
RWRWRWRWRWKRWRWRWRWRW
Physicochemical Analysis
C176H234N62O22
ANDCEQGHILMFPSTYV
RW
3570.20
13.36
11
0
10
+11
-9.41
-2.757
0.00
Mammalian: 1 hour Yeast: 2 min E.coli: 2 min
55000
1540.53
0
Residue Composition
number
0
A
10
R
0
N
0
D
0
C
0
E
0
Q
0
G
0
H
0
I
0
L
1
K
0
M
0
F
0
P
0
S
0
T
10
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 10 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 10 Y: 0 V: 0
Chemical Descriptors
21
C176H234N62O22
3570.20
13.36
+11
-9.41
-2.757
Free
L
Hybrid (Cyclic and Linear)
Conjugated with FITC
N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)O
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

CCRF-CEM, Sk-Ov-3, Hek-293 And MDA-MB-231 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Hybrid (Cyclic and Linear) L
Synthetic
Carboxyfluorescein-labeled phosphopeptide (F′-GpYEEI)
Cytosol
[(RW)5K](RW)5 (10 μM) enhanced the cellular uptake of F′-GpYEEI (2 μM) by 18-fold in CCRF-CEM cells.
Direct penetration and clathrin-mediated endocytosis
In vitro
CCCCSSCCCSSCSSCCSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

CCRF-CEM, Sk-Ov-3, Hek-293 And MDA-MB-231 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Hybrid (Cyclic and Linear) L
Synthetic
Carboxyfluorescein-labeled anti-HIV drugs (lamivudine (F′-3TC), emtricitabine (F′-FTC), Stavudine (F′-d4T))
Cytosol
[(RW)5K](RW)5 (10 μM) significantly increased the cellular uptake of F′-3TC, F′-FTC, F′-d4T (2μM) in CCRF-CEM by 4.3-, 7-, and 7.6-fold, respectively,
Direct penetration and clathrin-mediated endocytosis
In vitro
CCCCSSCCCSSCSSCCSCC
Additional Detail Fields 1 fields
CCCCSSCCCSSCSSCCSCC