TPDB

3D PDB MODEL

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Standard-residue backbone model; source-recorded chemical modifications are not verified in these coordinates.

TPDB ID TPDB63917

Peptide_Name

R9

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CellPPD-MOD CPPsite3.0 MLCPP 2.0 Peptipedia

Sequence_Type

modified_plain

Has_Modification Yes

Description A 10-aa modified cell-penetrating peptides peptide sequence curated from CellPPD-MOD, CPPsite3.0, MLCPP 2.0, and other sources, with an available 3D structural model.

Modification

C-terminal: Addition of Cysteine Chemical: Mal-PEG3400-b-PHEP3800 via covalent thiol-maleimide linkage

Sequence

RRRRRRRRRC

FASTA

>TPDB63917|Cell-penetrating Peptides|length=10
RRRRRRRRRC

Sequence-only estimates are not provided for modified or non-standard source notation. Reported molecular properties, when available, are retained below with their source records.

Length

10 reviewed residue count

C_terminal_Modification

Addition of Cysteine Activity

Chemical_Modification

Mal-PEG3400-b-PHEP3800 via covalent thiol-maleimide linkage Activity

Chirality

L Activity

Ionic_State

Cationic Activity

Linearity

Linear Activity

N_terminal_Modification

Free Activity

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)O Activity

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line 4T1 Cells

Assay & Model

In Vitro Model Female BALB/c mice (6~8 weeks)

In Vivo Model Female BALB/c mice (6~8 weeks)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Sensitive diblock copolymer PPC-DA and the insensitive PPC-SA conjugated DOX-nanoparticles

Uptake Efficiency

DA@R9-NPDOX at pH 6.5 were much stronger fluorescence signals of DOX than that at pH 7.4. In contrast, the enhanced fluorescence signals of DOX at pH 6.5 was not found for the SA@R9-NPDOX group.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCGGGTC

DSSP_Secondary_Structure

CCCCCGGGTC