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Peptide record

TPDB63863

[R5K]W5 Cell-penetrating Peptides standard
11 amino acids
Basic Information
3D PDB MODEL
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TPDB63863
[R5K]W5
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 11-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
Chemical: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)
Sequence
RRRRRKWWWWW
Physicochemical Analysis
C91H124N32O12
ANDCEQGHILMFPSTYV
RW
1858.19
13.00
6
0
5
+6
-4.21
-2.809
0.00
Mammalian: 1 hour Yeast: 2 min E.coli: 2 min
27500
1479.93
0
Residue Composition
number
0
A
5
R
0
N
0
D
0
C
0
E
0
Q
0
G
0
H
0
I
0
L
1
K
0
M
0
F
0
P
0
S
0
T
5
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 5 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 5 Y: 0 V: 0
Chemical Descriptors
11
C91H124N32O12
1858.19
13.00
+6
-4.21
-2.809
Free
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)
L
Amphipathic
Cyclic
Free
N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 1 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

MDA-MB-231, MDA-MB-468 , Llcpk And Au565 Wt Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Cyclic L Amphipathic
Synthetic
5-carboxyfluorescein (FAM)-labeled siRNA
Cytoplasm
In AUC65 cells, [R5K]W5 created higher mean fluorescence, both with and without DOPE (mean fluorescence readings of 1942, 921, and 1392 for N/P ratios of 20, 40, and 80 with DOPE, respectively, compared to 472, 611,and 1237 for corresponding N/P ratios for [R6K]W6)
In vitro
CCCCCCCSCC
Additional Detail Fields 1 fields
CCCCCCCSCC