TPDB

3D PDB MODEL

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Standard-residue backbone model; source-recorded chemical modifications are not verified in these coordinates.

TPDB ID TPDB63845

Peptide_Name

R4AANCK peptide

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

modified_plain

Has_Modification Yes

Description A 9-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

Chemical: Nanoparticles

Sequence

RRRRAANCK

FASTA

>TPDB63845|Cell-penetrating Peptides|length=9
RRRRAANCK

Sequence-only estimates are not provided for modified or non-standard source notation. Reported molecular properties, when available, are retained below with their source records.

Length

9 reviewed residue count

C_terminal_Modification

Free Activity

Chemical_Modification

Nanoparticles Activity

Chirality

L Activity

Linearity

Linear Activity

N_terminal_Modification

Free Activity

SMILES

N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)O Activity

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Du145 Cells

Assay & Model

In Vitro Model Female Rag2−/− mice (20–25 g)

In Vivo Model Female Rag2−/− mice (20–25 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Alexa Fluor 488-conjugated

Subcellular Localization Cytosol

Uptake Efficiency The CP50 values for R4 followed with concentrations of 3.2 μM at 60min incubation.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CBTTBTTCCCCHHHHHSCSSTTCC

DSSP_Secondary_Structure

CBTTBTTCCCCHHHHHSCSSTTCC