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Peptide record

TPDB63813

PEN0 PEN12 PEN4 Cell-penetrating Peptides standard
7 amino acids
Basic Information
3D PDB MODEL
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TPDB63813
PEN0 PEN12 PEN4
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CellPPD CellPPD-MOD CPPsite3.0 Peptipedia PerseuCPP
standard
No
A 7-aa standard-residue cell-penetrating peptides peptide sequence curated from CellPPD, CellPPD-MOD, CPPsite3.0, and other sources, with an available 3D structural model.
Sequence
RRMKWKK
Physicochemical Analysis

Sequence-only estimates for a linear peptide with free termini, not experimentally measured molecular properties. Mass and formula exclude cyclization and disulfide formation. The optical estimates assume paired cysteines, 280 nm, 1 g/L and a 1 cm path. The N-end rule estimate is not a measured plasma half-life.

C46H81N17O8S1
ANDCEQGHILFPSTYV
K
1032.32
12.43
5
0
2
+5
5.97
-2.814
0.00
Mammalian: 1 hour Yeast: 2 min E.coli: 2 min
5500
5.33
0
Residue Composition
number
0
A
2
R
0
N
0
D
0
C
0
E
0
Q
0
G
0
H
0
I
0
L
3
K
1
M
0
F
0
P
0
S
0
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 2 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 3 M: 1 F: 0 P: 0 S: 0 T: 0 W: 1 Y: 0 V: 0
Chemical Descriptors
7 reviewed residue count
C46H81N17O8S1 sequence-only estimate (linear, free termini)
1032.32 sequence-only estimate (linear, free termini)
12.43 sequence-only estimate (linear, free termini)
+5 sequence-only estimate (linear, free termini)
5.97 sequence-only estimate (linear, free termini)
-2.814 sequence-only estimate (linear, free termini)
Free Activity
L Activity
Cationic Activity
Linear Activity
Free Activity
N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CO)C(=O)O NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O Activity
Evidence Records 3 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Synthetic
pHPMA polymer
Cytoplasm
Internalized about 15 times more efficiently than the control polymer
Endocytosis
In vitro
CCBTTTBCSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
pHPMA polymer
Cytoplasm
Slightly outperformed TAT4
Endocytosis
In vitro
CCCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
pHPMA polymer
Cytoplasm
Two times higher penetration efficacy than the shorter analog PEN4
Endocytosis
In vitro
CCCCCCTTCC
Additional Detail Fields 1 fields
CCBTTTBCSCC CCCCC CCCCCCTTCC