TPDB

3D PDB MODEL

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Standard-residue backbone model; source-recorded chemical modifications are not verified in these coordinates.

TPDB ID TPDB63661

Peptide_Name

CPP33

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

modified_plain

Has_Modification Yes

Description A 16-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

C-terminal: Addition of Cysteine Chemical: Chimeric lipopepsomes (CLP)

Sequence

RLWMRWYSPRTRAYGC

FASTA

>TPDB63661|Cell-penetrating Peptides|length=16
RLWMRWYSPRTRAYGC

Sequence-only estimates are not provided for modified or non-standard source notation. Reported molecular properties, when available, are retained below with their source records.

Length

16 reviewed residue count

C_terminal_Modification

Addition of Cysteine Activity

Chemical_Modification

Chimeric lipopepsomes (CLP) Activity

Chirality

L Activity

Linearity

Linear Activity

N_terminal_Modification

Free Activity

SMILES

N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O Activity

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line A549 Cells ,MDA-MB-231 And Smmc-7721 Cells

Assay & Model

In Vitro Model A549 tumor–bearing nude mice

In Vivo Model A549 tumor–bearing nude mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Polo-like kinase 1 specific siRNA (siPLK1)

Uptake Efficiency

A549 human lung cancer cells had significantly higher cellular uptake of CPP33-CLP than other cancer cells including MDA-MB-231 and SMMC-7721 cells

Uptake Mechanism Dynamin-dependent and clathrin/micropinocytosis/caveolae-independent pathway

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCTTGGGCCCC

DSSP_Secondary_Structure

CCCCTTGGGCCCC