TPDB

3D PDB MODEL

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TPDB ID TPDB63660

Peptide_Name

RLW CPP33

Activity

Cell-penetrating Peptides Tumor-homing Peptides

Activity_category_statistics

Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0 MLCPP 2.0 Peptipedia PerseuCPP TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 15-aa standard natural multi-activity (Cell-penetrating Peptides and Tumor-homing Peptides) peptide sequence curated from CPPsite3.0, MLCPP 2.0, Peptipedia, and other sources, with an available 3D structural model.

Sequence

RLWMRWYSPRTRAYG

FASTA

>TPDB63660|Cell-penetrating Peptides;Tumor-homing Peptides|length=15
RLWMRWYSPRTRAYG

Formula C92H135N29O20S1

Absent Amino Acids NDCEQHIKFV

Common Amino Acids R

Mass 1999.33

PI 11.94

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 7

Net Charge +4

Boman Index -2.57

Hydrophobicity -1.227

Aliphatic Index 32.67

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 13980

Absorbance 280nm 699.24

Polar Residues 4

number

1

A

4

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

0

I

1

L

0

K

1

M

0

F

1

P

1

S

1

T

2

W

2

Y

0

V

Amino Acid Distribution

A: 1 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 1 K: 0 M: 1 F: 0 P: 1 S: 1 T: 1 W: 2 Y: 2 V: 0

Length

15

Formula

C92H135N29O20S1

Mass

1999.33

PI

11.94

Net Charge

+4

Boman Index

-2.57

Hydrophobicity

-1.227

C_terminal_Modification

Free

Chirality

L

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CO)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line

Many Human neoplastic cells including HeLa, Lovo, A549, MCF-7,MKN45,HepG2, LNCap, KPK,U2OS, RC-15,RD-ES,H28,K562,U251,NHDF

Assay & Model

In Vitro Model NOD-SCID mice model of xenograft human tumour cells

In Vivo Model NOD-SCID mice model of xenograft human tumour cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Fluorophore (FITC)

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCSSSCCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HUVEC and A549 cells

Assay & Model

In Vitro Model A549 xenografts bearing mice and U87 xenografts bearing mice

In Vivo Model A549 xenografts bearing mice and U87 xenografts bearing mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Nanoparticle (PEG-PCL)

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCSSSCCCCCCC

Evidence 3 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Homing or Targeting Homing

Target Tumor Lung cancer

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems

Year 2012

Reference

Eisaku Kondo et al. Nat Commun. 2012 Jul 17:3:951.

Other

Source Activity Label	Source Definition
Tumor-homing Peptides	TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.

InVitro ExVivo A549

InVivo Model 7-week-old NOD-SCID mouse

Source Type mRNA display library

DSSP_Secondary_Structure

CCCCCSSSCCCCCCC

InVitro_ExVivo

A549

InVivo_Model

7-week-old NOD-SCID mouse