TPDB

3D PDB MODEL

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TPDB ID TPDB63501

Peptide_Name

REDV-TAT-NLS-H12

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 34-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

Chemical: Micelles

Sequence

REDVYGRKKRRQRRRPKKKRKVHHHHHHHHHHHH

FASTA

>TPDB63501|Cell-penetrating Peptides|length=34
REDVYGRKKRRQRRRPKKKRKVHHHHHHHHHHHH

Formula C196H311N89O41

Absent Amino Acids ANCILMFSTW

Common Amino Acids H

Mass 4570.20

PI 12.42

Basic Residues 26

Acidic Residues 2

Hydrophobic Residues 3

Net Charge +13

Boman Index 27.43

Hydrophobicity -3.035

Aliphatic Index 17.06

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 32.60

Polar Residues 2

number

0

A

8

R

0

N

1

D

0

C

1

E

1

Q

1

G

12

H

0

I

0

L

6

K

0

M

0

F

1

P

0

S

0

T

0

W

1

Y

2

V

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 1 C: 0 E: 1 Q: 1 G: 1 H: 12 I: 0 L: 0 K: 6 M: 0 F: 0 P: 1 S: 0 T: 0 W: 0 Y: 1 V: 2

Length

34

Formula

C196H311N89O41

Mass

4570.20

PI

12.42

Net Charge

+13

Boman Index

27.43

Hydrophobicity

-3.035

C_terminal_Modification

Free

Chemical_Modification

Micelles

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HUVECs And Huasmcs Cells

Assay & Model

In Vitro Model Male mice (6 weeks old, 20–25 g)

In Vivo Model Male mice (6 weeks old, 20–25 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type pZNF583

Subcellular Localization Nucleus And Less In Lysosome

Uptake Efficiency Among all groups, REDV-TAT-NLS-H12/pZNF580 complexes group showed the best transfection result

Uptake Mechanism Clathrin-mediated and other endocytosis pathways

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCSSGGGGTCCCCCCSSSCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HUVECs And Huasmcs Cells

Assay & Model

In Vitro Model Male mice (6 weeks old, 20–25 g)

In Vivo Model Male mice (6 weeks old, 20–25 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Cy5-oligonucleotide complexes

Subcellular Localization Nucleus And Less In Lysosome

Uptake Efficiency MFI is nearly 14 folds higher than REDV-TAT-NLS-H0/Cy5 oligonucleotide complexe.

Uptake Mechanism Clathrin-mediated and other endocytosis pathways

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCSSGGGGTCCCCCCSSSCC

DSSP_Secondary_Structure

CCCCSSGGGGTCCCCCCSSSCC