TPDB

3D PDB MODEL

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TPDB ID TPDB63499

Peptide_Name

REDV-TAT-NLS-H4

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 26-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

Chemical: Micelles

Sequence

REDVYGRKKRRQRRRPKKKRKVHHHH

FASTA

>TPDB63499|Cell-penetrating Peptides|length=26
REDVYGRKKRRQRRRPKKKRKVHHHH

Formula C148H255N65O33

Absent Amino Acids ANCILMFSTW

Common Amino Acids R

Mass 3473.07

PI 12.42

Basic Residues 18

Acidic Residues 2

Hydrophobic Residues 3

Net Charge +12

Boman Index 19.75

Hydrophobicity -2.985

Aliphatic Index 22.31

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 42.90

Polar Residues 2

number

0

A

8

R

0

N

1

D

0

C

1

E

1

Q

1

G

4

H

0

I

0

L

6

K

0

M

0

F

1

P

0

S

0

T

0

W

1

Y

2

V

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 1 C: 0 E: 1 Q: 1 G: 1 H: 4 I: 0 L: 0 K: 6 M: 0 F: 0 P: 1 S: 0 T: 0 W: 0 Y: 1 V: 2

Length

26

Formula

C148H255N65O33

Mass

3473.07

PI

12.42

Net Charge

+12

Boman Index

19.75

Hydrophobicity

-2.985

C_terminal_Modification

Free

Chemical_Modification

Micelles

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HUVECs And Huasmcs Cells

Assay & Model

In Vitro Model Male mice (6 weeks old, 20–25 g)

In Vivo Model Male mice (6 weeks old, 20–25 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type pZNF581

Subcellular Localization Nucleus And Less In Lysosome

Uptake Efficiency All REDV-TAT-NLS-Hn/pZNF580 complexes could deliver pZNF580 into cells to express ZNF580 gene.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCSSGGGGGSCCCGGGCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HUVECs And Huasmcs Cells

Assay & Model

In Vitro Model Male mice (6 weeks old, 20–25 g)

In Vivo Model Male mice (6 weeks old, 20–25 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Cy5-oligonucleotide complexes

Subcellular Localization Nucleus And Less In Lysosome

Uptake Efficiency The MFI values increased along with Hn sequence length increased from 4 to 12.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCSSGGGGGSCCCGGGCCC

DSSP_Secondary_Structure

CCCCCSSGGGGGSCCCGGGCCC