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Peptide record

TPDB63362

TAT-S19 Cell-penetrating Peptides standard
30 amino acids
Basic Information
3D PDB MODEL
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TPDB63362
TAT-S19
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 30-aa standard-residue cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
Sequence
PFVIGAGVLGALGTGIGGIYGRKKRRQRRR
Physicochemical Analysis

Sequence-only estimates for a linear peptide with free termini, not experimentally measured molecular properties. Mass and formula exclude cyclization and disulfide formation. The optical estimates assume paired cysteines, 280 nm, 1 g/L and a 1 cm path. The N-end rule estimate is not a measured plasma half-life.

C142H245N51O34
NDCEHMSW
G
3210.83
12.71
8
0
11
+8
2.07
-0.307
91.00
Mammalian: >20 hour Yeast: >20 hour E.coli: ?
1490
0.46
3
Residue Composition
number
2
A
6
R
0
N
0
D
0
C
0
E
1
Q
8
G
0
H
3
I
2
L
2
K
0
M
1
F
1
P
0
S
1
T
0
W
1
Y
2
V
Amino Acid Distribution
A: 2 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 8 H: 0 I: 3 L: 2 K: 2 M: 0 F: 1 P: 1 S: 0 T: 1 W: 0 Y: 1 V: 2
Chemical Descriptors
30 reviewed residue count
C142H245N51O34 sequence-only estimate (linear, free termini)
3210.83 sequence-only estimate (linear, free termini)
12.71 sequence-only estimate (linear, free termini)
+8 sequence-only estimate (linear, free termini)
2.07 sequence-only estimate (linear, free termini)
-0.307 sequence-only estimate (linear, free termini)
Free Activity
L Activity
Linear Activity
Free Activity
N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O Activity
Evidence Records 1 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells And Lncap Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Fusogenic
Cy3-labelled siRNA and Cy3-labelled Ago2 fusion proteins
Cytoplasm
Cy3 fluorescence of TAT-S19-Ago2 in cells was higher than that of TAT-Ago2
Macropinocytosis
In vitro
CCIIIIIHHHHTSSSTTTTCCTTGGGTCCC
Additional Detail Fields 1 fields
CCIIIIIHHHHTSSSTTTTCCTTGGGTCCC