TPDB

3D PDB MODEL

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TPDB ID TPDB63273

Peptide_Name

NNJA_5

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 9-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Sequence

MTSKNTQIG

FASTA

>TPDB63273|Cell-penetrating Peptides|length=9
MTSKNTQIG

Formula C39H70N12O15S1

Absent Amino Acids ARDCEHLFPWYV

Common Amino Acids T

Mass 979.12

PI 10.10

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 2

Net Charge +1

Boman Index 1.87

Hydrophobicity -0.789

Aliphatic Index 43.33

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 5

number

0

A

0

R

1

N

0

D

0

C

0

E

1

Q

1

G

0

H

1

I

0

L

1

K

1

M

0

F

0

P

1

S

2

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 1 D: 0 C: 0 E: 0 Q: 1 G: 1 H: 0 I: 1 L: 0 K: 1 M: 1 F: 0 P: 0 S: 1 T: 2 W: 0 Y: 0 V: 0

Length

9

Formula

C39H70N12O15S1

Mass

979.12

PI

10.10

Net Charge

+1

Boman Index

1.87

Hydrophobicity

-0.789

C_terminal_Modification

Free

Chirality

L

Ionic_State

Hydrophobic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCSC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N1CCC[C@H]1C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hek-239 ,Caco2 Cells, SH-SY5Y Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Hydrophobic

Source of Peptide Phage Display

Cargo Type IgG4 antibody

Subcellular Localization Cytosol

Uptake Efficiency

Substantial levels of antibodies internalization in cells apart from CPP-12-AF488 peptide (no cargo attached) which showed no uptake after 3 hr and 24 hr incubation.

Uptake Mechanism Direct membrane translocation and Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hek, N2A And SH-SY5Y Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Hydrophobic

Source of Peptide Phage Display

Cargo Type siHPRT-Alexa647

Subcellular Localization Cytosol

Uptake Efficiency Internalization was observed with both conjugates with comparable level in HEK cells

Uptake Mechanism Direct membrane translocation and Endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCGGGSCC

DSSP_Secondary_Structure

CCCCCCCC CCGGGSCC