TPDB

3D PDB MODEL

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TPDB ID TPDB63259

Peptide_Name

MTD 1067

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 10-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Sequence

MRAAAPAVAA

FASTA

>TPDB63259|Cell-penetrating Peptides|length=10
MRAAAPAVAA

Formula C39H69N13O11S1

Absent Amino Acids NDCEQGHILKFSTWY

Common Amino Acids A

Mass 928.12

PI 11.05

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +1

Boman Index 0.08

Hydrophobicity 1.080

Aliphatic Index 89.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

6

A

1

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

0

K

1

M

0

F

1

P

0

S

0

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 6 R: 1 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 1 F: 0 P: 1 S: 0 T: 0 W: 0 Y: 0 V: 1

Length

10

Formula

C39H69N13O11S1

Mass

928.12

PI

11.05

Net Charge

+1

Boman Index

0.08

Hydrophobicity

1.080

C_terminal_Modification

Free

Chirality

L

Ionic_State

Hydrophobic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCSC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Artificial Skin Fdc-P1,Nih 3T3, B16F10,Hdfa And Hacat Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Hydrophobic

Source of Peptide Synthetic

Cargo Type FITC-labelled GHRP-6 peptide

Uptake Efficiency Permeability of FITC-labeled MTD-GHRP-6 was increased by approximately five times that of GHRP-6

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCTTC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Artificial Skin Fdc-P1,Nih 3T3, B16F10,Hdfa And Hacat Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Hydrophobic

Source of Peptide Synthetic

Cargo Type FITC-labelled des(1-3)IGF-I peptide

Uptake Efficiency MTD-des(1-3)IGF-I showed 14 times higher fluorescence intensity in the dermal layer than des(1-3)IGF-I

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSGGGCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Artificial Skin Fdc-P1,Nih 3T3, B16F10,Hdfa And Hacat Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Hydrophobic

Source of Peptide Synthetic

Cargo Type Rhodamine-labeled PDGF-BB peptide

Uptake Efficiency Rhodamine-labeled MTD-PDGF-BB also showed higher fluorescence approximately 32 times compared to PDGF-BB

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSGGGCC

DSSP_Secondary_Structure

CCCCTTC CCCSSGGGCC