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Peptide record

TPDB63176

pHK-PASA488d Cell-penetrating Peptides modified_plain modified
22 amino acids
Basic Information
3D PDB MODEL
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Standard-residue backbone model; source-recorded chemical modifications are not verified in these coordinates.

TPDB63176
pHK-PASA488d
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
modified_plain
Yes
A 22-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
C-terminal: Amidation N-terminal: Conjugated with Alexa Fluor 488
Sequence
MIASHLLAYFFTELNGKPILFF-NH2
Physicochemical Analysis

Sequence-only estimates are not provided for modified or non-standard source notation. Reported molecular properties, when available, are retained below with their source records.

Chemical Descriptors
22 reviewed residue count
Amidation Activity
L Activity
Hydrophobic Activity
Linear Activity
Conjugated with Alexa Fluor 488 Activity
N[C@@H](CCSC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)O Activity
Evidence Records 1 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, CHO-K1, Or Pgsa-745 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Hydrophobic
Chimeric
Alexa 488 dye
Cytosol and Mitochondria
Enhancement in uptake of pHK-PASA488 relative to pHKA488 ranged from 2.9-fold at 5 mM to 3.5-fold at 50 mM in HeLa cells. At 10mM in CHO-K1, or pgsA-745 cell lines, uptake is comparable, while at 25 and 50 mM pHK-PASA488, uptake in the mutant pgsA-745 cells was 64 6 3 and 80 6 3%, respectively, of that of the uptake in the parent CHO-K1 cells.
Energy-independent mechanisms and macropinocytosis
In vitro
CCSSCHIIIIISCC
Additional Detail Fields 1 fields
CCSSCHIIIIISCC