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Peptide record

TPDB63176

pHK-PASA488d Cell-penetrating Peptides standard
22 amino acids
Basic Information
3D PDB MODEL
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TPDB63176
pHK-PASA488d
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 22-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
C-terminal: Amidation N-terminal: Conjugated with Alexa Fluor 488
Sequence
MIASHLLAYFFTELNGKPILFF-NH2
Physicochemical Analysis
C127H186N26O29S1
RDCQWV
LF
2573.09
7.75
2
1
14
+0
-3.77
1.000
115.45
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
1490
57.91
4
Residue Composition
number
2
A
0
R
1
N
0
D
0
C
1
E
0
Q
1
G
1
H
2
I
4
L
1
K
1
M
4
F
1
P
1
S
1
T
0
W
1
Y
0
V
Amino Acid Distribution
A: 2 R: 0 N: 1 D: 0 C: 0 E: 1 Q: 0 G: 1 H: 1 I: 2 L: 4 K: 1 M: 1 F: 4 P: 1 S: 1 T: 1 W: 0 Y: 1 V: 0
Chemical Descriptors
22
C127H186N26O29S1
2573.09
7.75
+0
-3.77
1.000
Amidation
L
Hydrophobic
Linear
Conjugated with Alexa Fluor 488
N[C@@H](CCSC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)O
Evidence Records 1 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, CHO-K1, Or Pgsa-745 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Hydrophobic
Chimeric
Alexa 488 dye
Cytosol and Mitochondria
Enhancement in uptake of pHK-PASA488 relative to pHKA488 ranged from 2.9-fold at 5 mM to 3.5-fold at 50 mM in HeLa cells. At 10mM in CHO-K1, or pgsA-745 cell lines, uptake is comparable, while at 25 and 50 mM pHK-PASA488, uptake in the mutant pgsA-745 cells was 64 6 3 and 80 6 3%, respectively, of that of the uptake in the parent CHO-K1 cells.
Energy-independent mechanisms and macropinocytosis
In vitro
CCSSCHIIIIISCC
Additional Detail Fields 1 fields
CCSSCHIIIIISCC