TPDB

3D PDB MODEL

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Standard-residue backbone model; source-recorded chemical modifications are not verified in these coordinates.

TPDB ID TPDB63147

Peptide_Name

AA3H-PLP

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

modified_plain

Has_Modification Yes

Description A 22-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

Chemical: Protein linking peptide (PLP) N-terminal: Conjugated with FITC

Sequence

MASIWVGHRGRRRQQQQQQRRR

FASTA

>TPDB63147|Cell-penetrating Peptides|length=22
MASIWVGHRGRRRQQQQQQRRR

Sequence-only estimates are not provided for modified or non-standard source notation. Reported molecular properties, when available, are retained below with their source records.

Length

22 reviewed residue count

C_terminal_Modification

Free Activity

Chemical_Modification

Protein linking peptide (PLP) Activity

Chirality

L Activity

Linearity

Linear Activity

N_terminal_Modification

Conjugated with FITC Activity

SMILES

N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O Activity

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells And Fibroblasts Cells

Assay & Model

In Vitro Model Five weeks-old BALB/c mice

In Vivo Model Five weeks-old BALB/c mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Chimeric

Cargo Type β-galactosidase and Cre recombinase

Uptake Efficiency

β-galactosidase delivered by AA3H produced 298pg/mL of o-nitrophenol, a 6-fold higher amount than obtained with the enzyme delivered by TAT (47pg/mL). AA3H-conjugated Cre exhibited a 2.1-times higher RFP expression level than cells treated with the TAT-conjugated enzyme

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSCCSSCCHHHHHHHHHHTC

DSSP_Secondary_Structure

CCSCCSSCCHHHHHHHHHHTC