TPDB

3D PDB MODEL

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Standard-residue backbone model; source-recorded chemical modifications are not verified in these coordinates.

TPDB ID TPDB63099

Peptide_Name

R8MTS

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

modified_plain

Has_Modification Yes

Description A 25-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

C-terminal: Addition of Cysteine Chemical: N-(2-hydroxypropyl) methacrylamide (HPMA) ploymer

Sequence

LSRTRAAAPNSRIFTRRRRRRRRRC

FASTA

>TPDB63099|Cell-penetrating Peptides|length=25
LSRTRAAAPNSRIFTRRRRRRRRRC

Sequence-only estimates are not provided for modified or non-standard source notation. Reported molecular properties, when available, are retained below with their source records.

Length

25 reviewed residue count

C_terminal_Modification

Addition of Cysteine Activity

Chemical_Modification

N-(2-hydroxypropyl) methacrylamide (HPMA) ploymer Activity

Chirality

L Activity

Linearity

Linear Activity

N_terminal_Modification

Free Activity

SMILES

N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)O Activity

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line 4T1 And MDA-MB-231 Cells

Assay & Model

In Vitro Model Female BALB/c mice (4-6 weeks)

In Vivo Model Female BALB/c mice (4-6 weeks)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Doxorubicin (DOX)

Subcellular Localization Mitochondria

Uptake Efficiency P-D-R8MTS showed enhanced cellular uptake with 1.51-fold higher than PD at 4 h.

Uptake Mechanism More than one endocytosis pathways including macropinocytosis

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSCTTGGGHHHHHHHTTSCCC

DSSP_Secondary_Structure

CCSCTTGGGHHHHHHHTTSCCC