TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB62859

Peptide_Name

DL-1 DL-2

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 11-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Sequence

KWSSKKSKHCG

FASTA

>TPDB62859|Cell-penetrating Peptides|length=11
KWSSKKSKHCG

Formula C55H90N18O15S1

Absent Amino Acids ARNDEQILMFPTYV

Common Amino Acids K

Mass 1275.49

PI 10.55

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 1

Net Charge +4

Boman Index 3.23

Hydrophobicity -1.818

Aliphatic Index 0.00

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 431.21

Polar Residues 4

number

0

A

0

R

0

N

0

D

1

C

0

E

0

Q

1

G

1

H

0

I

0

L

4

K

0

M

0

F

0

P

3

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 1 E: 0 Q: 0 G: 1 H: 1 I: 0 L: 0 K: 4 M: 0 F: 0 P: 0 S: 3 T: 0 W: 1 Y: 0 V: 0

Length

11

Formula

C55H90N18O15S1

Mass

1275.49

PI

10.55

Net Charge

+4

Boman Index

3.23

Hydrophobicity

-1.818

C_terminal_Modification

Free

Chirality

L

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)NCC(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)O.CCCCN

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Abdominal Skin From Male Kunming (Km) Mice

Assay & Model

In Vitro Model Male Kunming (KM) mice

In Vivo Model Male Kunming (KM) mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Triptolide (TP)

Uptake Efficiency No change in accumulative penetration amount of TP

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Abdominal Skin From Male Kunming (Km) Mice

Assay & Model

In Vitro Model Male Kunming (KM) mice

In Vivo Model Male Kunming (KM) mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Triptolide (TP)

Uptake Efficiency Accumulative penetration amount of TP is increased

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCHHHHHHHTSCSSSCCCC

DSSP_Secondary_Structure

CCCSSSTTCCCTTTTC CCCHHHHHHHTSCSSSCCCC