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Peptide record

TPDB62805

CPP44 Cell-penetrating Peptides Tumor-homing Peptides standard
15 amino acids
Basic Information
3D PDB MODEL
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TPDB62805
CPP44
Cell-penetrating Peptides Tumor-homing Peptides
Cancer-related peptides Delivery and barrier-penetrating peptides
CPPsite3.0 MLCPP 2.0 Peptipedia PerseuCPP TumorHoPe2.0
standard
No
A 15-aa standard natural multi-activity (Cell-penetrating Peptides and Tumor-homing Peptides) peptide sequence curated from CPPsite3.0, MLCPP 2.0, Peptipedia, and other sources, with an available 3D structural model.
Sequence
KRPTMRFRYTWNPMK
Physicochemical Analysis
C91H142N28O20S2
ADCEQGHILSV
R
2012.42
12.14
5
0
5
+5
1.63
-1.667
0.00
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
6990
347.34
4
Residue Composition
number
0
A
3
R
1
N
0
D
0
C
0
E
0
Q
0
G
0
H
0
I
0
L
2
K
2
M
1
F
2
P
0
S
2
T
1
W
1
Y
0
V
Amino Acid Distribution
A: 0 R: 3 N: 1 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 2 M: 2 F: 1 P: 2 S: 0 T: 2 W: 1 Y: 1 V: 0
Chemical Descriptors
15
C91H142N28O20S2
2012.42
12.14
+5
1.63
-1.667
Free
L
Linear
Free
N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC(=O)N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 3 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Many Human neoplastic cells including HeLa, Lovo, A549, MCF-7,MKN45,HepG2, LNCap, KPK,U2OS, RC-15,RD-ES,H28,K562,U251,NHDF

Assay & Model

NOD-SCID mice model of xenograft human tumour cells
NOD-SCID mice model of xenograft human tumour cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Synthetic
Fluorophore (FITC)
primary myelogenous leukaemic cells (AML) showed uptake of CPP44, but not of TAT or CPP2 at comparable concentrations, showed poor penetration into normal fibroblasts
In vitro and in vivo
CCCCCCCCCCCCTTC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HEK293T cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L
Synthetic
Cre protein
Cytoplasm
Lower degree of Cre delivery
In vitro
Evidence 3 Activity

Activity

Tumor-homing Peptides

Target

Homing
Myelogenous leukemia, Hepatoblastoma

Source & Reference

TumorHoPe2.0
TumorHoPe2
Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems
2012
Eisaku Kondo et al. Nat Commun. 2012 Jul 17:3:951.

Other

Source Activity Label Source Definition
Tumor-homing Peptides
TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.
K562, HepG2, HAK-1B9, HLE, AML (multiple patients)
7-week-old NOD-SCID mouse
mRNA display library
CPP44-RI-p16MIS, FITC-CPP44
Additional Detail Fields 4 fields
CPP44-RI-p16MIS, FITC-CPP44
CCCCCCCCCCCCTTC
K562, HepG2, HAK-1B9, HLE, AML (multiple patients)
7-week-old NOD-SCID mouse