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Peptide record

TPDB62591

(KH)9-Bp100 KH9-BP100 Cell-penetrating Peptides standard
29 amino acids
Basic Information
3D PDB MODEL
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TPDB62591
(KH)9-Bp100 KH9-BP100
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 MLCPP 2.0 Peptipedia PerseuCPP
standard
No
A 29-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, MLCPP 2.0, Peptipedia, and other sources, with an available 3D structural model.
Sequence
KHKHKHKHKHKHKHKHKHKKLFKKILKYL
Physicochemical Analysis
C180H295N61O31
ARNDCEQGMPSTWV
K
3809.71
11.30
23
0
6
+15
18.44
-2.276
53.79
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
1490
39.11
1
Residue Composition
number
0
A
0
R
0
N
0
D
0
C
0
E
0
Q
0
G
9
H
1
I
3
L
14
K
0
M
1
F
0
P
0
S
0
T
0
W
1
Y
0
V
Amino Acid Distribution
A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 9 I: 1 L: 3 K: 14 M: 0 F: 1 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0
Chemical Descriptors
29
C180H295N61O31
3809.71
11.30
+15
18.44
-2.276
Free
L
Cationic
Linear
Free
N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)NCC(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)O.CCCCN NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)O
Evidence Records 6 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Arabidopsis thaliana leaf cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Synthetic
Nucleic acid (ds RNA)
In vitro
CCSCCCCCCCTTHHHHTTTC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

A. Thaliana Leaves

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Fusogenic
GFP-expressing plasmid DNA and YFP-antisense dsRNA
Cytosol
Rapid GFP production in 12hr
In vitro
CCSCCCCCCCTTHHHHTTTC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

A. Thaliana Leaves ,N. Benthamiana Leaf And Tomato (Solanum Lycopersicum (S. Lycopersicum) And Solanum Tuberosum)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Fusogenic
Cyanine‐3 (Cy3) labeled pDNA (pPpsbA::Rluc)
Cytoplasm and Chloroplasts
BP100‐KH9 or KH9‐BP100 as CPP did not improve the chloroplast transfection efficiency in A. thaliana
In vitro
CCCHHHHHHHTSCSSSCCCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

Nicotiana Benthamiana Leaves

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Fusogenic
pDNA of psfGN155-MxMT and psfGC155-MxMT
Cytosol and Nucleus
High levels of accumulation of both sfGN155-MxMT and sfGC155-MxMT proteins with pDNA/CPP complexes
In vitro
CHHHHHHHHHTTTCCCGGGTCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

Nicotiana Benthamiana Leaves

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Fusogenic
ssRNA of psfGN155-MxMT and psfGC155-MxMT
Cytosol and Nucleus
ssRNA/CPP complexes were significantly higher than the free ssRNA levels in leaf cells.
In vitro
CCSCCCCCCCTTHHHHTTTC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

Tobacco (Nicotiana Tabacum Cv. Petit Havana Sr1) And Rice (Oryza Sativa Cv. Nipponbare)

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L
Fusogenic
pGWB-Nluc
In vitro
Additional Detail Fields 1 fields
CCSCCCCCCCTTHHHHTTTC CCCHHHHHHHTSCSSSCCCC CHHHHHHHHHTTTCCCGGGTCC