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Peptide record

TPDB62587

KH27K Cell-penetrating Peptides standard
29 amino acids
Basic Information
3D PDB MODEL
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TPDB62587
KH27K
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 29-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
C-terminal: Amidation N-terminal: Acetylation
Sequence
Ac-KHHHHHHHHHHHHHHHHHHHHHHHHHHHK-NH2
Physicochemical Analysis
C174H215N85O30
ARNDCEQGILMFPSTWYV
H
3977.17
10.60
29
0
0
+4
27.90
-3.248
0.00
Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min
0
0.00
0
Residue Composition
number
0
A
0
R
0
N
0
D
0
C
0
E
0
Q
0
G
27
H
0
I
0
L
2
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 27 I: 0 L: 0 K: 2 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
29
C174H215N85O30
3977.17
10.60
+4
27.90
-3.248
Amidation
L
Amphipathic
Linear
Acetylation
N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CS)C(=O)O.C(C)C
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Human Embryonic Kidney-Derived Cells 293Tt , 3T6 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Amphipathic
Protein derived
VLPs*(MPyV virus-like particles labeled with Alexa Fluor 488)
High relative MFI for VLP*/KH27K than control VLPs*
Macropinocytosis
In vitro
CCSCHHHHCCCSSCSCCSSSCSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Human Embryonic Kidney-Derived Cells 293Tt , 3T6 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphipathic
Protein derived
PsVs of MPyV(SV40 and MusPV)
Nucleus
KH27K consistently enhanced transduction by all PsVs tested (3-fold for MPyV PsVs and 6-fold for SV40 at 20 μM), albeit MusPV PsVs transduction was potentiated only 2.5-fold. Internalization for all VLP*/CPP complexes shows Low relative MFI except for VLP*/KH27K in comparison to control VLPs* (without peptide) in 3T6 cells.
Endocytosis
In vitro
CCSCHHHHCCCSSCSCCSSSCSCC
Additional Detail Fields 1 fields
CCSCHHHHCCCSSCSCCSSSCSCC