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Peptide record

TPDB62509

L17E Cell-penetrating Peptides standard
25 amino acids
Basic Information
3D PDB MODEL
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TPDB62509
L17E
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 25-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
C-terminal: Amidation
Sequence
IWLTALKFLGKHAAKHEAKQQLSKL-NH2
Physicochemical Analysis
C134H219N37O32
RNDCMPYV
LK
2860.44
10.71
7
1
12
+4
3.56
-0.228
109.60
Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour
5500
192.28
4
Residue Composition
number
4
A
0
R
0
N
0
D
0
C
1
E
2
Q
1
G
2
H
1
I
5
L
5
K
0
M
1
F
0
P
1
S
1
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 4 R: 0 N: 0 D: 0 C: 0 E: 1 Q: 2 G: 1 H: 2 I: 1 L: 5 K: 5 M: 0 F: 1 P: 0 S: 1 T: 1 W: 1 Y: 0 V: 0
Chemical Descriptors
25
C134H219N37O32
2860.44
10.71
+4
3.56
-0.228
Amidation
L
Cationic and amphiphilic Amphiphilic
Linear
Free
N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)O N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)O
Evidence Records 3 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells , Sw480 Cells, Cos-7 Cells And Nih3T3 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic and amphiphilic
Synthetic
Dextran and IgG- labelled with Alexa Fluor 488
Cytoplasm and nucleus
Almost 50% of the cells co-incubated with L17E (40 μM) showed spread signals of Alexa488–dextran in the cytosol and in the nucleus
Macropinocytosis
In vitro
CCCSHHHHHHSCCSSHHHHHSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
Alexa568-labeled IgG (25 μg/mL)
Cytosol
In vitro
CCSCTTSGGGGTCCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Nih3T3 And Hek293 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Amphiphilic
Synthetic
Alexa Fluor 488-labelled Dex11
Cytoplasm
≈50 % cells with 40 μm L17E
Endocytosis
In vitro
CCCHHHHHHHTTSCCC
Additional Detail Fields 1 fields
CCCSHHHHHHSCCSSHHHHHSCC CCSCTTSGGGGTCCCC CCCHHHHHHHTTSCCC