TPDB

3D PDB MODEL

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TPDB ID TPDB62445

Peptide_Name

A22p

Activity

Cell-penetrating Peptides Tumor-homing Peptides

Activity_category_statistics

Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0 TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 22-aa standard natural multi-activity (Cell-penetrating Peptides and Tumor-homing Peptides) peptide sequence curated from CPPsite3.0 and TumorHoPe2.0, with an available 3D structural model.

Sequence

HTPGNSNKWKHLQENKKGRPRR

FASTA

>TPDB62445|Cell-penetrating Peptides;Tumor-homing Peptides|length=22
HTPGNSNKWKHLQENKKGRPRR

Formula C114H186N44O31

Absent Amino Acids ADCIMFYV

Common Amino Acids K

Mass 2669.01

PI 12.16

Basic Residues 9

Acidic Residues 1

Hydrophobic Residues 2

Net Charge +6

Boman Index 10.95

Hydrophobicity -2.527

Aliphatic Index 17.73

Half Life

Mammalian: 3.5 hour Yeast: 10 min E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 206.07

Polar Residues 6

number

0

A

3

R

3

N

0

D

0

C

1

E

1

Q

2

G

2

H

0

I

1

L

4

K

0

M

0

F

2

P

1

S

1

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 3 N: 3 D: 0 C: 0 E: 1 Q: 1 G: 2 H: 2 I: 0 L: 1 K: 4 M: 0 F: 0 P: 2 S: 1 T: 1 W: 1 Y: 0 V: 0

Length

22

Formula

C114H186N44O31

Mass

2669.01

PI

12.16

Net Charge

+6

Boman Index

10.95

Hydrophobicity

-2.527

C_terminal_Modification

Free

Chirality

L

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Ai9 Tdtomato Npcs

Assay & Model

In Vitro Model Ai9 or wild-type mice

In Vivo Model Ai9 or wild-type mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Protein derived

Cargo Type SpyCas9 RNPs

Uptake Efficiency >3-fold improvements in genome editing compared to the reference SV40 NLS.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSSSCCTTTSCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Homing or Targeting Targeting

Receptors Biomarker NRP-b1

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title

Enhancement of the tumor penetration of monoclonal antibody by fusion of a neuropilin-targeting peptide improves the antitumor efficacy

Year 2014

Reference

Shin T H et al. Mol Cancer Ther.2014 Mar 13(3):651-61

Other

Source Activity Label	Source Definition
Tumor-homing Peptides	TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.

InVitro ExVivo

Human umbilical vein endothelial cells (HUVEC), Human cancer cell line, SK-OV-3 (Ovary), A431 (Epidermis), FaDu (Pharynx)

InVivo Model Female BALB/c athymic nude mice

Source Type Synthetic

DSSP_Secondary_Structure

CCSSSCCTTTSCCCCCC

InVitro_ExVivo

Human umbilical vein endothelial cells (HUVEC), Human cancer cell line, SK-OV-3 (Ovary), A431 (Epidermis), FaDu (Pharynx)

InVivo_Model

Female BALB/c athymic nude mice