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Peptide record

TPDB62421

NF 71 Cell-penetrating Peptides modified_plain modified
22 amino acids
Basic Information
TPDB62421
NF 71
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
modified_plain
Yes
A 22-aa modified cell-penetrating peptides peptide sequence curated from CPPsite3.0, without an available 3D structural model.
C-terminal: Amidation Chemical: Extracellular vesicles (EVs) N-terminal: Stearoylation
Sequence
HHYHHGO*ILLKALKALAKAIL-NH2
Physicochemical Analysis
C107H175N31O22
RNDCEQMFPSTWV
L
2247.76
10.46
7
0
12
+3
1.78
0.409
142.27
Mammalian: 3.5 hour Yeast: 10 min E.coli: >10 hour
1490
66.29
1
Residue Composition
number
4
A
0
R
0
N
0
D
0
C
0
E
0
Q
1
G
4
H
2
I
5
L
3
K
0
M
0
F
0
P
0
S
0
T
0
W
1
Y
0
V
Amino Acid Distribution
A: 4 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 4 I: 2 L: 5 K: 3 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0
Chemical Descriptors
22
C107H175N31O22
2247.76
10.46
+3
1.78
0.409
Amidation
Extracellular vesicles (EVs)
Modified
Linear
Stearoylation
Evidence Records 1 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

CHO-K1, N2A, Hek293, And A549 Cell Lines

Assay & Model

Male and female 8-week-old BALB/c mice
Male and female 8-week-old BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Non-natural residues Linear Modified
Firefly luciferase-encoding plasmid (pMC.BESPX-GLucFLuc2) and CD63 EGFP-encoding plasmid (AddGene pEGFP C2 62964)
Complexes using NF55 or NF71 shows no significant difference in the levels of luciferase reporter protein.
In vitro and in vivo