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Peptide record

TPDB62281

HIV-1 TAT peptide--Crystallins TAT TAT-BoNTA Cell-penetrating Peptides standard
12 amino acids
Basic Information
3D PDB MODEL
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TPDB62281
HIV-1 TAT peptide--Crystallins TAT TAT-BoNTA
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 Peptipedia PerseuCPP
standard
No
A 12-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, Peptipedia, and PerseuCPP, with an available 3D structural model.
Sequence
GRKKRRQRRRPQ
Physicochemical Analysis
C65H124N34O15
ANDCEHILMFSTWYV
R
1621.92
13.10
8
0
0
+8
8.46
-3.650
0.00
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
2
Residue Composition
number
0
A
6
R
0
N
0
D
0
C
0
E
2
Q
1
G
0
H
0
I
0
L
2
K
0
M
0
F
1
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 2 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 1 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
12
C65H124N34O15
1621.92
13.10
+8
8.46
-3.650
Free
L
Cationic
Linear
Free
NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)O N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 7 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HLE B3 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Protein derived
Protein (Human B-Crystallin)
in vitro
CCCCCCCCCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Jurkat T Cells And U937 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
FITC-labelled
Cytoplasm and nucleus
In vitro
CCCCCCCCCCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

Hek293T And Ct26.Cl25 Cell Lines

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
5-methoxyuridine-modified mCherry mRNA, Enhanced green fluorescence protein (EGFP) mRNA and Ovalbumin (OVA) mRNA
Cytoplasm
Exhibited poor uptake efficiency with 6.57% for TAT
In vitro
CCCCCCCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

Hek-293T Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Mannosyl erythritol lipid (MEL)-based peptide linker modified Texas red-DHPE-encapsulated LPNVs
Cytosol
The LPNVTat and LPNVArg7 only surrounded the ER when they were dispersed in the cytosol, implying that Tat and Arg7 could lead the NVs towards the ER and nucleus but did not assist their penetration through the ER membrane.
In vitro
CCCCCCGGGCCSSCCCCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

Hepg2 Cells

Assay & Model

Xenograft tumor bearing male nude mice weighing 18–20 g were
Xenograft tumor bearing male nude mice weighing 18–20 g were

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Gold nanorods (AuNRs)
Significantly larger cellular uptake of Au@Res in the presence of additional MMP-9 in the media, reaching 3.3 times more Au concentration in the cells after 24 h.
In vitro and in vivo
CCSCCSSCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

N2A Cells

Assay & Model

Eight week old, C57BL/6J female mice (17–22 g)
Eight week old, C57BL/6J female mice (17–22 g)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Botulinum neurotoxin serotype A (BoNTA)
Exhibiting highest improvement of cellular uptake
In vitro and in vivo
CCCCCSCCCC
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Target

A431 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
7D12 anti-EGFR nanobody
Total fluorescence intensity was significantly lower than 7D12-Atto532.
In vitro
CCCCCTTTTCCGGGSSSSCC
Additional Detail Fields 1 fields
CCCCCCCCCCCC CCCCCCCC CCCCCCGGGCCSSCCCCC CCSCCSSCC CCCCCSCCCC CCCCCTTTTCCGGGSSSSCC