TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB62262

Peptide_Name

NP2 (TAT) NP3 (TAT)

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 12-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Modification

Chemical: Rhodamine fluorophore were conjugated to 2-maleimido-dextran nanoparticles N-terminal: Thiolated

Sequence

GRKKRRQRRRPK

FASTA

>TPDB62262|Cell-penetrating Peptides|length=12
GRKKRRQRRRPK

Formula C66H128N34O14

Absent Amino Acids ANDCEHILMFSTWYV

Common Amino Acids R

Mass 1621.96

PI 13.10

Basic Residues 9

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +9

Boman Index 8.87

Hydrophobicity -3.683

Aliphatic Index 0.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

0

A

6

R

0

N

0

D

0

C

0

E

1

Q

1

G

0

H

0

I

0

L

3

K

0

M

0

F

1

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 1 G: 1 H: 0 I: 0 L: 0 K: 3 M: 0 F: 0 P: 1 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

12

Formula

C66H128N34O14

Mass

1621.96

PI

13.10

Net Charge

+9

Boman Index

8.87

Hydrophobicity

-3.683

C_terminal_Modification

Free

Chemical_Modification

Rhodamine fluorophore were conjugated to 2-maleimido-dextran nanoparticles

Chirality

L

Linearity

Linear

N_terminal_Modification

Thiolated

SMILES

N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](Cc1[nH]cnc1)C(=O)NCC(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa-Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Chimeric

Cargo Type 3.6 copies of peptidomimetic inhibitor 1

Uptake Efficiency Efficient cellular uptake was detected for NP2

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCTTGGGGCSCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa-Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Chimeric

Cargo Type 6 copies of peptidomimetic inhibitor 1

Uptake Efficiency Efficient cellular uptake was detected for NP3

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCCCCC

DSSP_Secondary_Structure

CCCTTGGGGCSCCCC CCCCCCCCCCCC