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Peptide record

TPDB62207

C-Peptide HBD Cell-penetrating Peptides standard
27 amino acids
Basic Information
3D PDB MODEL
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TPDB62207
C-Peptide HBD
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0
standard
No
A 27-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.
Sequence
GPGLWERQAREHSERKKRRRESECKAA
Physicochemical Analysis
C134H224N52O41S1
NDIMFTYV
R
3251.64
10.77
10
5
5
+4
17.04
-2.078
25.56
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
5500
169.15
4
Residue Composition
number
3
A
6
R
0
N
0
D
1
C
5
E
1
Q
2
G
1
H
0
I
1
L
3
K
0
M
0
F
1
P
2
S
0
T
1
W
0
Y
0
V
Amino Acid Distribution
A: 3 R: 6 N: 0 D: 0 C: 1 E: 5 Q: 1 G: 2 H: 1 I: 0 L: 1 K: 3 M: 0 F: 0 P: 1 S: 2 T: 0 W: 1 Y: 0 V: 0
Chemical Descriptors
27
C134H224N52O41S1
3251.64
10.77
+4
17.04
-2.078
Free
L
Linear
Free
NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)O N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(=O)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)O
Evidence Records 2 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

HeLa, Bcap-37, A549, 95-D, 5637, A357, Bxpc-3 , Mgc80-3, Mkn45, T24, Mrc-5, Cos-7, L02, Ok And CHO Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Protein derived
EGFP protein or FITC
Cytoplasm and nucleus
MGC80-3 and MKN45 presented a high degree of fusion protein transduction, while 5637 and A549 cells shows resistance to fusion protein penetration. Transmembrane efficiency of HBD-EGFP is improved about 10 times compared with that of EGFP-HBD
Energy-dependent direct translocation
In vitro
CCCSTHHHHSSSTTTTTCCTTSSSCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

MDA-MB-231 Cell Cancer Cell Lines (HeLa, Hep G2, A549, MCF-7) And Normal Breast Cells (Nhfb).

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
EGFP and VPR proteins
Cytoplasm
The C peptide increased the EGFP uptake in all tested cells, while SP90 and SP90-C only improved the uptake of EGFP in breast cancer cells.
Macropinocytosis
In vitro
CCCHHHHHHTTC
Additional Detail Fields 1 fields
CCCSTHHHHSSSTTTTTCCTTSSSCC CCCHHHHHHTTC