TPDB

3D PDB MODEL

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TPDB ID TPDB61858

Peptide_Name

F4R8 (Alexa) F4R8--p27kip1C F4R8-PAD

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CellPPD-MOD CPPsite3.0 Peptipedia

Sequence_Type

standard

Has_Modification No

Description A 15-aa standard natural cell-penetrating peptides peptide sequence curated from CellPPD-MOD, CPPsite3.0, and Peptipedia, with an available 3D structural model.

Modification

C-terminal: Amidation

Sequence

FFFFGRRRRRRRRGC-NH2

FASTA

>TPDB61858|Cell-penetrating Peptides|length=15
FFFFGRRRRRRRRGC-NH2

Formula C91H145N39O16S1

Absent Amino Acids ANDEQHILKMPSTWYV

Common Amino Acids R

Mass 2073.46

PI 12.88

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 4

Net Charge +8

Boman Index 1.74

Hydrophobicity -1.540

Aliphatic Index 0.00

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

0

A

8

R

0

N

0

D

1

C

0

E

0

Q

2

G

0

H

0

I

0

L

0

K

0

M

4

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 0 C: 1 E: 0 Q: 0 G: 2 H: 0 I: 0 L: 0 K: 0 M: 0 F: 4 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

15

Formula

C91H145N39O16S1

Mass

2073.46

PI

12.88

Net Charge

+8

Boman Index

1.74

Hydrophobicity

-1.540

C_terminal_Modification

Amidation

Chirality

L

Ionic_State

Cationic

Linearity

Linear

SMILES

N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CS)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, T98G glioma cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (Alexa)

Subcellular Localization Cytosol

Uptake Efficiency Uptake was higher in comparison to PasR8

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCTTCCTTHHHHTTC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, T98G glioma cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Peptide (cyclin-dependent kinase inhibitor)

Subcellular Localization Cytosol

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCTTCCTTHHHHTTC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, T98G glioma cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Peptide [(Proapoptotic domain (PAD)]

Subcellular Localization Cytosol

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCTTCCTTHHHHTTC

DSSP_Secondary_Structure

CCTTCCTTHHHHTTC