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Peptide record

TPDB61463

LDP-NLS HR9 Cell-penetrating Peptides standard
21 amino acids
Basic Information
3D PDB MODEL
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TPDB61463
LDP-NLS HR9
Cell-penetrating Peptides
Delivery and barrier-penetrating peptides
CPPsite3.0 MLCPP 2.0 Peptipedia
standard
No
A 21-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, MLCPP 2.0, and Peptipedia, with an available 3D structural model.
Sequence
CHHHHHRRRRRRRRRHHHHHC
Physicochemical Analysis
C120H190N68O22S2
ANDEQGILKMFPSTWYV
H
3001.39
12.70
19
0
0
+10
16.41
-3.214
0.00
Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour
125
4.16
2
Residue Composition
number
0
A
9
R
0
N
0
D
2
C
0
E
0
Q
0
G
10
H
0
I
0
L
0
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 9 N: 0 D: 0 C: 2 E: 0 Q: 0 G: 0 H: 10 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
21
C120H190N68O22S2
3001.39
12.70
+10
16.41
-3.214
Free
L
Cationic Amphipathic
Linear
Free
N[C@@H](CS)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CS)C(=O)O N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)O NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 8 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

A549 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Synthetic
Nucleic acid (Plasmid DNA)
Direct membrane translocation
In vitro
CCHHHHGGGCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

Hek-293T Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
plasmid DNAs (pEGFP-NS3)
The uptake efficiency of the complexes was in the order of +36GFP/NS3 DNA>TurboFect/NS3 DNA>HR9/NS3 DNA complexes
In vitro
CCHHHHGGGCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Assay & Model

6-8 week old female BALB/c mice
6-8 week old female BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Synthetic
Protein and DNA (Nucleic acid) encoding T-cell epitopes
In vivo
CCHHHHGGGCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

A549 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Cationic
Synthetic
Carboxyl-functionalized CdSe/ZnS QDs eFluor 625NC (red luminescent QD)
HR9-mediated transduction remained higher than transduction mediated by SR9 and PR9 for 4 h
In vitro
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

Hek-293T Cells

Assay & Model

5 to 7 week-old female BALB/c mice
5 to 7 week-old female BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
DNA (Nef DNA, Hsp20-Nef DNA)
Effectively delivered DNA
In vitro and in vivo
CCBTTTBCSCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

Hek-293 T Cells

Assay & Model

Five to seven week-old female BALB/c mice
Five to seven week-old female BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Amphipathic
Synthetic
NS3 and Hsp27- NS3 DNA nanoparticles
Deliver pEGFPHsp27-NS3 with the transfection rates of about 33.34% ± 0.94
In vitro and in vivo
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Assay & Model

Six to eight-week-old female BALB/c mice
Six to eight-week-old female BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Synthetic
In vivo
CCCSTHHHHHHHHHHHTTCCSSSCTHHHHHHHHCC
Evidence 8 Activity

Activity

Cell-penetrating Peptides

Assay & Model

Six to eight-week-old female BALB/c mice
Six to eight-week-old female BALB/c mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Non-natural residues Linear L Cationic
Chimeric
In vivo
Additional Detail Fields 1 fields
CCHHHHGGGCC CCBTTTBCSCC CCCSTHHHHHHHHHHHTTCCSSSCTHHHHHHHHCC