TPDB

Activity

Cell-penetrating Peptides

Activity_category_statistics

Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 11-aa standard natural cell-penetrating peptides peptide sequence curated from CPPsite3.0, with an available 3D structural model.

Sequence

CGRKKRRQRRR

FASTA

>TPDB61425|Cell-penetrating Peptides|length=11
CGRKKRRQRRR

Formula C58H114N32O13S1

Absent Amino Acids ANDEHILMFPSTWYV

Common Amino Acids R

Mass 1499.81

PI 12.71

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +8

Boman Index 7.19

Hydrophobicity -3.291

Aliphatic Index 0.00

Half Life

Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 2

number

0

A

6

R

0

N

0

D

1

C

0

E

1

Q

1

G

0

H

0

I

0

L

2

K

0

M

0

F

0

P

0

S

0

T

0

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 6 N: 0 D: 0 C: 1 E: 0 Q: 1 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

11

Formula

C58H114N32O13S1

Mass

1499.81

PI

12.71

Net Charge

+8

Boman Index

7.19

Hydrophobicity

-3.291

C_terminal_Modification

Free

Chirality

L

Ionic_State

Cationic

Linearity

Linear Cyclic

N_terminal_Modification

Free

SMILES

N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CS)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, Kb , MCF-7 And CHO Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type FITC-labelled Quantum dot (QD)

Subcellular Localization Cytosol Towards Golgi Apparatus

Uptake Efficiency

QD(TAT)10 need at least 1 h for significant labelling and uptake also uptake kinetics of QD(TAT)10 is slower than QD(TAT)75

Uptake Mechanism Lipid-raft mediated endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCTTCCGGGGGGGCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, Kb , MCF-7 And CHO Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type FITC-labelled Quantum dot (QD)

Subcellular Localization Cytosol Towards Golgi Apparatus

Uptake Efficiency QD(TAT)20 need at least 1 h for significant labelling and uptake

Uptake Mechanism Lipid-raft mediated endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSGGGCCSCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, Kb , MCF-7 And CHO Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type FITC-labelled Quantum dot (QD)

Subcellular Localization Cytosol Towards Exocytosis

Uptake Efficiency QD(TAT)40 or QD(TAT)75 can label cells within 30 min

Uptake Mechanism Exocytosized and lipid-raft-mediated endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSGGGCCSCC

Evidence 4 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, Kb , MCF-7 And CHO Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type FITC-labelled Quantum dot (QD)

Subcellular Localization Cytosol Towards Exocytosis

Uptake Efficiency QD(TAT)40 or QD(TAT)75 can label cells within 30 min

Uptake Mechanism Exocytosized and lipid-raft-mediated endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSGGGCCSCC

Evidence 5 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line CHO-K1, CHO-Pgsa745

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Cyclic L Cationic

Source of Peptide Synthetic

Cargo Type Protein kinase C inhibitor (PKCi)

Subcellular Localization Cytoplasm

Uptake Efficiency Moderate improve in cellular uptake of cyclic CPP

Uptake Mechanism GAG-dependent endocytosis and Direct translocation

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCHHHHHHHTTSCHHHHTTTTTTSCC

DSSP_Secondary_Structure

CCTTCCGGGGGGGCCCC CCSGGGCCSCC CCHHHHHHHTTSCHHHHTTTTTTSCC