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Peptide record

TPDB61409

tLyp-1 tLyp1 tLyP‑1 Cell-penetrating Peptides Tumor-homing Peptides standard

7 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB61409

Peptide_Name

tLyp-1 tLyp1 tLyP‑1

Activity

Cell-penetrating Peptides Tumor-homing Peptides

Activity_category_statistics

Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

CPPsite3.0 Peptipedia PerseuCPP TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 7-aa standard natural multi-activity (Cell-penetrating Peptides and Tumor-homing Peptides) peptide sequence curated from CPPsite3.0, Peptipedia, PerseuCPP, and other sources, with an available 3D structural model.

Sequence

CGNKRTR

FASTA

>TPDB61409|Cell-penetrating Peptides;Tumor-homing Peptides|length=7
CGNKRTR

Formula C31H59N15O10S1

Absent Amino Acids ADEQHILMFPSWYV

Common Amino Acids R

Mass 833.96

PI 11.32

Basic Residues 3

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +3

Boman Index 2.94

Hydrophobicity -2.143

Aliphatic Index 0.00

Half Life

Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 3

number

Amino Acid Distribution

A: 0 R: 2 N: 1 D: 0 C: 1 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 0 K: 1 M: 0 F: 0 P: 0 S: 0 T: 1 W: 0 Y: 0 V: 0

Length

Formula

C31H59N15O10S1

Mass

833.96

11.32

Net Charge

Boman Index

2.94

Hydrophobicity

-2.143

C_terminal_Modification

Free

Chirality

Linearity

Linear

Motif

CendR (R/KXXR/K) CendR (R/K)XX(R/K) CendR (R/KXXXR/K)

N_terminal_Modification

Free

SMILES

N[C@@H](CS)C(=O)NCC(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HUVEC cells and C6 glioma cells

Assay & Model

In Vitro Model Balb/c nude mice

In Vivo Model Balb/c nude mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Nanoparticle, and Fluorophore (Couramin-6)

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CTTCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line U87 Cells

Assay & Model

In Vitro Model U87 glioma bearing male BALB/c nude mice

In Vivo Model U87 glioma bearing male BALB/c nude mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L

Source of Peptide Synthetic

Cargo Type NP- siRNA(siLSINCT5) conjugated with anti-NKG2A monoclonal antibody (aNKG2A)

Uptake Efficiency tLypNP-siRNA displayed an obvious higher fluorescent signal than the cells treated by undecorated NP-siRNA

Uptake Mechanism Actin-, caveolae-, energy-mediated endocytosis and lysosomes.

In Vitro or In Vivo In vitro and in vivo

Evidence 3 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor

Lung cancer, Breast cancer, Liver cancer, Melanoma, stomach cancer, Pancreatic cancer, Colorectal cancer, Ovarian cancer, Renal cell carcinoma, Prostate cancer, Brain tumor

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Noxa-derived, cell death-inducing peptide eMTD

Year 2022

Reference

US 11248024 B2

Patent US 11248024 B2

Other

Source Activity Label	Source Definition
Tumor-homing Peptides	TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.

InVitro ExVivo Sp2/0, CHO (Chinese hamster ovary) K1, CHO DG44, PER.C6, W138, BHK, COS7, 293, HepG2, Huh7, 3T3, RIN, MDCK cell line

InVivo Model 6-week-old BalB/C mice

Source Type Synthetic

Conjugate Payload necrosis-inducing peptide, Fluorescein (FAM)

Evidence 4 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor lymphatic, Tumor cell, Tumor macrophage, Endothelial cell

Homing or Targeting Homing

Target Tumor Breast cancer, Prostate cancer

Receptors Biomarker neuropilin-1, neuropilin-2

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Truncated LYP-1 peptides and methods and compositions using truncated LYP-1 peptides

Year 2019

Reference

US 10179801 B2

Patent US 10179801 B2

Other

InVitro ExVivo 4T1, MDA-MB-435 Breast cancer cell line, DU145, PPC1

InVivo Model BALB/C athymic nude mice

Source Type Phage Display library

Phage Display Library T7 phage display library

Conjugate Payload fluorescein-labeled, iron oxide nanoworms

Evidence 5 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor Colorectal cancer

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title

Application of the Tumor Site Recognizable and Dual-Responsive Nanoparticles for Combinational Treatment of the Drug-Resistant Colorectal Cancer

Year 2020

Reference

Ying Shi et al. Pharm Res. 2020 Mar 25. 37(4):72.

Other

InVitro ExVivo HCT-15cells andHUVECcells

InVivo Model The male BALB/c nude mice, weight of ~20 g and age of 4–5 weeks

Source Type Synthetic

Conjugate Payload mPEG-PLA, Paclitaxel

Evidence 6 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Parenchyma cell, Tumor cell, Endothelial cell, Tumor vessel

Homing or Targeting Homing

Target Tumor Glioma

Receptors Biomarker neuropilin 1 (NRP-1)

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Co-administration of dual-targeting nanoparticles with penetration enhancement peptide for antiglioblastoma therapy

Year 2014

Reference

Deyu Miao et al. Mol Pharm. 2014 Jan 6 11(1):90-101.

Other

InVitro ExVivo BCEC and C6cells

InVivo Model Male BALB/c nude mice weighing 20 ± 2 g, male Spraguee−Dawley (SD) rats weighing 200 ± 20 g

Source Type Synthetic

Conjugate Payload paclitaxel, Lf-functionalizednanoparticles(Lf-NP)

Evidence 7 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor Renal cell carcinoma (RCC)

Receptors Biomarker Neuropilin-1 and Neuropilin-2 (NRP1/2)

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Dual Targeting of Endoplasmic Reticulum by Redox-Deubiquitination Regulation for Cancer Therapy

Year 2021

Reference

Biao Cai et al. Int J Nanomedicine. 2021 Jul 30:16:5193-5209.

Other

InVitro ExVivo

786-O renal cell carcinoma cells, HK-2 normal renal tubular epithelial cells, PC-3 prostate cancer cells, Human umbilical vein endothelial cells (HUVECs)

InVivo Model Male BALB/c nude mice (4–6 weeks old)

Source Type Synthetic

Conjugate Payload PR-619 (DUB inhibitor), Fe3O4 nanoparticles (NPs)

Evidence 8 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor Triple negative Breast cancer

Receptors Biomarker Neuropilin-1 (NRP-1), CD44

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title

A dual receptors-targeting and size-switchable "cluster bomb" co-loading chemotherapeutic and transient receptor potential ankyrin 1 (TRPA-1) inhibitor for treatment of triple negative breast cancer

Year 2020

Reference

Yashi Wang et al. J Control Release. 2020 May 10:321:71-83.

Other

InVitro ExVivo 4T1 murine breast cancer cells

InVivo Model Balb/c mice (6–8 weeks old, 18–22 g)

Conjugate Payload Doxorubicin (DOX), AP-18, DSPE-PEG2000, hyaluronic acid nanogels (HA-NG)

Evidence 9 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title

Addition of free poloxamer 407 to a new gene vector P407-PEI-K12 solution forms a sustained-release in situ hypergel that enhances cell transfection and extends gene expression

Year 2019

Reference

HONGMEI SHU et al. Oncol Lett. 2019 Mar 17. (3) 3085-3096

Other

Source Type Synthetic

Evidence 10 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell, endothelial cell

Homing or Targeting Homing

Target Tumor Glioma (Brain tumor)

Receptors Biomarker neuropilin-1 (NRP1)

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title F3 peptide-functionalized PEG-PLA nanoparticles co-administrated with tLyp-1 peptide for anti-glioma drug delivery

Year 2012

Reference

Quanyin Hu et al. Biomaterials. 2013 Jan 34(4):1135-45.

Other

InVitro ExVivo Rat C6 glioma cell line

InVivo Model Male Sprague-Dawley rats (200 20 g), Balb/c nude mice (male, 4e5 weeks, 20 2g)

Source Type Synthetic

Conjugate Payload Nanoparticles (pegylated poly (trimethylene carbonate))

Evidence 11 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell, Endothelial cell

Homing or Targeting Homing

Target Tumor Glioma

Receptors Biomarker neuropilin- 1

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Glioma therapy using tumor homing and penetrating peptide-functionalized PEG-PLA nanoparticles loaded with paclitaxel

Year 2013

Reference

Quanyin Hu et al. Biomaterials. 2013 Jul 34(22):5640-50.

Other

InVitro ExVivo human umbilical vein endothelial cells and Rat C6 glioma cells

Source Type Synthetic

Conjugate Payload PEG-PLA nanoparticles, paclitaxel

Evidence 12 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor Breast cancer

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Tumor homing-penetrating and nanoenzyme-augmented 2D phototheranostics against hypoxic solid tumors

Year 2022

Reference

Chunhui Wu et al. Acta Biomater. 2022 Sep 15:150:391-401.

Other

InVitro ExVivo 4T1 cell line

Conjugate Payload chlorin e6 (Ce6)

Comments penetrating

Evidence 13 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor Breast cancer

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Dual-targeting exosomes for improved drug delivery in breast cancer

Year 2023

Reference

Nam Hb Tran et al. Nanomedicine (Lond). 2023 Mar 18(7):599-611.

Other

InVitro ExVivo MCF-7and MDA-MB-231 breast cancer cell line

Source Type Phage Display Library

Conjugate Payload doxorubicin (Dox)

Evidence 14 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Homing or Targeting Homing

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Year 2023

Reference

Ludivine Larue et al. Bioorg Chem. 2023 Jan:130:106200.

Evidence 15 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor endothelial cell, tumor cell

Homing or Targeting Homing

Target Tumor Breast cancer, Prostate cancer, Glioma

Receptors Biomarker Neuropilin (NRP)

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title The penetrating properties of the tumor homing peptide LyP-1 in model lipid membranes

Year 2019

Reference

Cionabasu C et al. J Pept Sci. 2019 Mar 25(3):e3145.

Other

Source Type Synthetic

Conjugate Payload Dy647P1,Streptavidin, Biotin

Evidence 16 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor Multiple tumor

Receptors Biomarker Neuropilin-1

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Stimuli-Responsive "Cluster Bomb" for Programmed Tumor Therapy

Year 2017

Reference

Lei Q et al. ACS Nano. 2017 Jul 25 11(7):7201-7214.

Other

InVitro ExVivo 4T1

InVivo Model Female BALB/C mice (5-6 week) injected subcutaneously with 4T1 cells

Source Type Synthetic

Conjugate Payload Doxorubicin, FITC, Tungsten Sulfide Quantum Dots (WS2 QDs), Mesoporous silica nanoparticle (MSN)

Comments Adamantane (Ad) modification attached to lysine (K) residue at position 1 of the peptide sequence

Evidence 17 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing

Target Tumor Breast cancer

Receptors Biomarker NRP-1, NRP-2

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title A smart viral vector for targeted delivery of hydrophobic drugs

Year 2021

Reference

Ghosh S et al. Sci Rep. 2021 Mar 29 11(1):7030

Other

InVitro ExVivo MDA-MB-231

Source Type Synthetic

Conjugate Payload Virus Like Particles(VHPs) of Flock House Virus

Evidence 18 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Homing or Targeting Homing

Target Tumor Brain tumor

Receptors Biomarker Neuropilin-1

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Tumor-targeting dual peptides-modified cationic liposomes for delivery of siRNA and docetaxel to gliomas

Year 2014

Reference

Yang Z Z et al. Biomaterials.2014 Jun 35(19):5226-39

Other

InVitro ExVivo

U251 MG cells (human glioblastoma cells), C6 cells (rat glioma cells), U87 MG cells (human glioblastoma cells), BMVEC (murine brain microvascular endothelial cells)

InVivo Model Male BALB/c nude mice

Source Type Synthetic

Conjugate Payload Polyethylene glycol (PEG)

Evidence 19 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Target Cell Tumor cell

Homing or Targeting Homing, Penetrating

Receptors Biomarker Neuropilin-1

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title tLyP-1-conjugated Au-nanorod@SiO(2) core-shell nanoparticles for tumor-targeted drug delivery and photothermal therapy

Year 2014

Reference

Xu B et al. Langmuir.2014 Jul 8 30(26):7789-97

Other

InVitro ExVivo Hela cells, MCF-7 cells, hMSCs, MG63 cells

Source Type Synthetic

Conjugate Payload AuNR@SiO2, Fluorescein isothiocyanate (FITC)

Evidence 20 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Homing or Targeting Homing

Target Tumor Breast cancer

Receptors Biomarker NRP-1

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title

Peptide-Functionalized Phase-Transformation Nanoparticles for Low Intensity Focused Ultrasound-Assisted Tumor Imaging and Therapy

Year 2018

Reference

Zhu L et al. Nano Lett. 2018 Mar 14 18(3):1831-1841.

Other

InVitro ExVivo MDA-MB-231

InVivo Model Nude mice bearing MDA-MB-231 xenograft

Source Type Phage display library

Conjugate Payload 10-Hydroxycamptothecin (10-HCPT), peﬂuoropentane (PFP)

Evidence 21 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Homing or Targeting Targeting

Receptors Biomarker Neuropilins

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title

Design of a dendrimer with a matrix metalloproteinase-responsive fluorescence probe and a tumor-homing peptide for metastatic tumor cell imaging in the lymph node

Year 2021

Reference

Nagai K et al. ioorg Med Chem Lett.2021 Feb 1:33:127726

Other

InVitro ExVivo HT-1080(human fibrosarcoma cells), 4T1(mouse mammary tumor cells), RAW264(mouse macrophage like cells)

Source Type Synthetic

Conjugate_Payload

necrosis-inducing peptide, Fluorescein (FAM) fluorescein-labeled, iron oxide nanoworms mPEG-PLA, Paclitaxel paclitaxel, Lf-functionalizednanoparticles(Lf-NP) PR-619 (DUB inhibitor), Fe3O4 nanoparticles (NPs) Doxorubicin (DOX), AP-18, DSPE-PEG2000, hyaluronic acid nanogels (HA-NG) Nanoparticles (pegylated poly (trimethylene carbonate)) PEG-PLA nanoparticles, paclitaxel chlorin e6 (Ce6) doxorubicin (Dox) Dy647P1,Streptavidin, Biotin Doxorubicin, FITC, Tungsten Sulfide Quantum Dots (WS2 QDs), Mesoporous silica nanoparticle (MSN) Virus Like Particles(VHPs) of Flock House Virus Polyethylene glycol (PEG) AuNR@SiO2, Fluorescein isothiocyanate (FITC) 10-Hydroxycamptothecin (10-HCPT), peﬂuoropentane (PFP)

DSSP_Secondary_Structure

CTTCCCC

InVitro_ExVivo

Sp2/0, CHO (Chinese hamster ovary) K1, CHO DG44, PER.C6, W138, BHK, COS7, 293, HepG2, Huh7, 3T3, RIN, MDCK cell line 4T1, MDA-MB-435 Breast cancer cell line, DU145, PPC1 HCT-15cells andHUVECcells BCEC and C6cells 786-O renal cell carcinoma cells, HK-2 normal renal tubular epithelial cells, PC-3 prostate cancer cells, Human umbilical vein endothelial cells (HUVECs) 4T1 murine breast cancer cells Rat C6 glioma cell line human umbilical vein endothelial cells and Rat C6 glioma cells 4T1 cell line MCF-7and MDA-MB-231 breast cancer cell line 4T1 MDA-MB-231 U251 MG cells (human glioblastoma cells), C6 cells (rat glioma cells), U87 MG cells (human glioblastoma cells), BMVEC (murine brain microvascular endothelial cells) Hela cells, MCF-7 cells, hMSCs, MG63 cells HT-1080(human fibrosarcoma cells), 4T1(mouse mammary tumor cells), RAW264(mouse macrophage like cells)

InVivo_Model

6-week-old BalB/C mice BALB/C athymic nude mice The male BALB/c nude mice, weight of ~20 g and age of 4–5 weeks Male BALB/c nude mice weighing 20 ± 2 g, male Spraguee−Dawley (SD) rats weighing 200 ± 20 g Male BALB/c nude mice (4–6 weeks old) Balb/c mice (6–8 weeks old, 18–22 g) Male Sprague-Dawley rats (200 20 g), Balb/c nude mice (male, 4e5 weeks, 20 2g) Female BALB/C mice (5-6 week) injected subcutaneously with 4T1 cells Male BALB/c nude mice Nude mice bearing MDA-MB-231 xenograft

Phage_Display_Library

T7 phage display library